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A more recent version of this article appeared on June 1, 2004
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Submitted on October 29, 2003
Revised on March 15, 2004
Accepted on March 17, 2004
1 University of Connecticut Health Center, Department of Physiology and Center for Biomedical Imaging Technology, 263 Farmington Avenue - MC1507, Farmington, CT 06032-1507
* Corresponding author. E-mail address: rodionov{at}nso.uchc.edu.
Numerous evidence demonstrates that dynein is crucial for organization of microtubules (MT) into radial arrays, but its exact function in this process is unclear. Here we studied the role of cytoplasmic dynein in MT radial array formation in the absence of the centrosome. We found that dynein is a potent MT nucleator in vitro and that stimulation of dynein activity in cytoplasmic fragments of melanophores induces nucleation-dependent formation of MT radial array in the absence of the centrosome. This new property of dynein, in combination with its known role as a MT motor that is essential for MT array organization in the absence and presence of the centrosome, makes it a unique molecule whose activity is necessary and sufficient for the formation and maintenance of MT radial arrays in cells.
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