Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 14, 2004
Mol. Biol. Cell 10.1091/mbc.E03-12-0875

A more recent version of this article appeared on October 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-12-0875v1
15/10/4369    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gorsich, S. W.
Right arrow Articles by Shaw, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gorsich, S. W.
Right arrow Articles by Shaw, J. M.

Submitted on December 8, 2003
Revised on July 2, 2004
Accepted on July 6, 2004

The Importance of Mitochondrial Dynamics During Meiosis and Sporulation

Steven W. Gorsich* and Janet M. Shaw{dagger}

Department of Biology, University of Utah, Salt Lake City, UT 84112

Monitoring Editor: Thomas Fox

Opposing fission and fusion events maintain the yeast mitochondrial network. Six proteins regulate these membrane dynamics during mitotic growth. Dnm1p, Mdv1p and Fis1p mediate fission. Fzo1p, Mgm1p, and Ugo1p mediate fusion. Previous studies established that mitochondria fragment and rejoin at distinct stages during meiosis and sporulation, suggesting that mitochondrial fission and fusion are required during this process. Here we report that strains defective for mitochondrial fission alone, or both fission and fusion, complete meiosis and sporulation. However, visualization of mitochondria in sporulating cultures reveals morphological defects associated with the loss of fusion and/or fission proteins. Specifically, mitochondria collapse to one side of the cell and fail to fragment during pre-sporulation. In addition, mitochondria are not inherited equally by newly formed spores, and mtDNA nucleoid segregation defects give rise to spores lacking nucleoids. This nucleoid inheritance defect is correlated with an increase in petite spore colonies. Unexpectedly, mitochondria fragment in mature tetrads lacking fission proteins. The latter finding suggests that: 1) a novel fission machinery operates during sporulation, or 2) mechanical forces generate the mitochondrial fragments observed in mature spores. These results provide evidence of fitness defects caused by fission mutations and reveal new phenotypes associated with fission and fusion mutations.

*Present address: USDA Agricultural Research Service, Peoria, IL 61604

{dagger}Corresponding author. E-mail: shaw{at}bioscience.utah.edu




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
N. Nakamura and S. Hirose
Regulation of Mitochondrial Morphology by USP30, a Deubiquitinating Enzyme Present in the Mitochondrial Outer Membrane
Mol. Biol. Cell, May 1, 2008; 19(5): 1903 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. Wells, L. K. Picton, S. C. P. Williams, F. J. Tan, and R. B. Hill
Direct Binding of the Dynamin-like GTPase, Dnm1, to Mitochondrial Dynamics Protein Fis1 Is Negatively Regulated by the Fis1 N-terminal Arm
J. Biol. Chem., November 16, 2007; 282(46): 33769 - 33775.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Y. Suda, H. Nakanishi, E. M. Mathieson, and A. M. Neiman
Alternative Modes of Organellar Segregation during Sporulation in Saccharomyces cerevisiae
Eukaryot. Cell, November 1, 2007; 6(11): 2009 - 2017.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Lee, S.-Y. Jeong, W.-C. Lim, S. Kim, Y.-Y. Park, X. Sun, R. J. Youle, and H. Cho
Mitochondrial Fission and Fusion Mediators, hFis1 and OPA1, Modulate Cellular Senescence
J. Biol. Chem., August 3, 2007; 282(31): 22977 - 22983.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Taguchi, N. Ishihara, A. Jofuku, T. Oka, and K. Mihara
Mitotic Phosphorylation of Dynamin-related GTPase Drp1 Participates in Mitochondrial Fission
J. Biol. Chem., April 13, 2007; 282(15): 11521 - 11529.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Nakada, A. Sato, K. Yoshida, T. Morita, H. Tanaka, S.-I. Inoue, H. Yonekawa, and J.-I. Hayashi
Mitochondria-related male infertility
PNAS, October 10, 2006; 103(41): 15148 - 15153.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Durr, M. Escobar-Henriques, S. Merz, S. Geimer, T. Langer, and B. Westermann
Nonredundant Roles of Mitochondria-associated F-Box Proteins Mfb1 and Mdm30 in Maintenance of Mitochondrial Morphology in Yeast
Mol. Biol. Cell, September 1, 2006; 17(9): 3745 - 3755.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
K. S. Dimmer and L. Scorrano
(De)constructing Mitochondria: What For?
Physiology, August 1, 2006; 21: 233 - 241.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Neutzner and R. J. Youle
Instability of the Mitofusin Fzo1 Regulates Mitochondrial Morphology during the Mating Response of the Yeast Saccharomyces cerevisiae
J. Biol. Chem., May 13, 2005; 280(19): 18598 - 18603.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.