![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on May 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 16, 2004
Revised on February 23, 2004
Accepted on February 24, 2004
1 MRC - Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK, Contributed equally
2 Applied Optics and Information Processing, Kirchoff Institute for Physics, University of Heidelberg, Im Neuenheimer Feld 227, 69120 Heidelberg, Germany; Physics of Condensed Matter, Institute of Physics, University of Potsdam, Am Neuen Palais 10, 14469 Potsdam, Germany, Contributed equally
3 Applied Optics and Information Processing, Kirchoff Institute for Physics, University of Heidelberg, Im Neuenheimer Feld 227, 69120 Heidelberg, Germany
4 MRC - Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK
* Corresponding author. E-mail address: ana.pombo{at}csc.mrc.ac.uk.
Spatially modulated illumination fluorescence microscopy can in theory measure the sizes of objects with a diameter ranging between 10-200 nm, and has allowed accurate size measurement of subresolution fluorescent beads (
40-100 nm). Biological structures in this size range have so far been measured by electron microscopy. Here we have labeled sites containing the active, hyperphosphorylated form of RNA polymerase II in the nucleus of HeLa cells using the antibody H5. The spatially modulated illumination microscope was compared with confocal laser scanning and electron microscopes and found to be suitable for measuring the size of cellular nanostructures in a biological setting. The hyperphosphorylated form of polymerase II was found in structures with 70 nm diameter well below the 200 nm-resolution limit of standard fluorescence microscopes.
This article has been cited by other articles:
|
|
 |
|
  J. Huve, R. Wesselmann, M. Kahms, and R. Peters 4Pi Microscopy of the Nuclear Pore Complex Biophys. J., July 15, 2008; 95(2): 877 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. Eskiw, A. Rapp, D. R. F. Carter, and P. R. Cook RNA polymerase II activity is located on the surface of protein-rich transcription factories J. Cell Sci., June 15, 2008; 121(12): 1999 - 2007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Shopland, C. R. Lynch, K. A. Peterson, K. Thornton, N. Kepper, J. v. Hase, S. Stein, S. Vincent, K. R. Molloy, G. Kreth, et al. Folding and organization of a contiguous chromosome region according to the gene distribution pattern in primary genomic sequence J. Cell Biol., July 3, 2006; 174(1): 27 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Michalet and S. Weiss Using photon statistics to boost microscopy resolution PNAS, March 28, 2006; 103(13): 4797 - 4798. [Full Text] [PDF]
|
|
|
|
|
|
S. M. Gorisch, M. Wachsmuth, K. F. Toth, P. Lichter, and K. Rippe Histone acetylation increases chromatin accessibility J. Cell Sci., December 15, 2005; 118(24): 5825 - 5834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. N. Day and F. Schaufele Imaging Molecular Interactions in Living Cells Mol. Endocrinol., July 1, 2005; 19(7): 1675 - 1686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hildenbrand, A. Rapp, U. Spori, C. Wagner, C. Cremer, and M. Hausmann Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy Biophys. J., June 1, 2005; 88(6): 4312 - 4318. [Abstract] [Full Text] [PDF]
|
|
