Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print June 23, 2004
Mol. Biol. Cell 10.1091/mbc.E04-01-0058

A more recent version of this article appeared on September 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Figure
Right arrow All Versions of this Article:
E04-01-0058v1
15/9/4148    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deora, A. A.
Right arrow Articles by Rodriguez-Boulan, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deora, A. A.
Right arrow Articles by Rodriguez-Boulan, E.

Submitted on January 21, 2004
Revised on May 28, 2004
Accepted on June 14, 2004

The Basolateral Targeting Signal of CD147 (EMMPRIN) Consists of a Single Leucine and Is Not Recognized by Retinal Pigment Epithelium

Ami A. Deora*, Diego Gravotta*, Geri Kreitzer{dagger}, Jane Hu{ddagger}, Dean Bok{ddagger}{sect}||, and Enrique Rodriguez-Boulan*¶

*Margaret M. Dyson Vision Research Institute, Department of Ophthalmology, {dagger}Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10021; and {ddagger}Jules Stein Eye Institute, {sect}Brain Research Institute and ||Department of Neurobiology, School of Medicine, University of California, Los Angeles, CA 90095

Monitoring Editor: Juan S. Bonifacino

CD147, a type I integral membrane protein of the immunoglobulin superfamily, exhibits reversed polarity in Retinal Pigment Epithelium (RPE). CD147 is apical in RPE in contrast to its basolateral localization in extraocular epithelia. This elicited our interest in understanding the basolateral sorting signals of CD147 in prototypic Madin-Darby Canine Kidney (MDCK) cells. The cytoplasmic domain of CD147 has basolateral sorting information but is devoid of well-characterized basolateral signals, such as tyrosine and di-leucine motifs. Hence, we carried out systematic site-directed mutagenesis to delineate basolateral targeting information in CD147. Our detailed analysis identified a single leucine (252) as the basolateral targeting motif in the cytoplasmic tail of CD147. Four amino acids (243-246) N-terminal to leucine 252 are also critical basolateral determinants of CD147, since deletion of these amino acids leads to mistargeting of CD147 to the apical membranes. We ruled out the involvement of adaptor complex 1B (AP1B) in the basolateral trafficking of CD147, since LLC-PK1 cells lacking AP1B, target CD147 basolaterally. At variance with MDCK cells, the human RPE cell line ARPE-19 does not distinguish between CD147 (WT) and CD147 with leucine 252 mutated to alanine and targets both proteins apically. Thus, our study identifies an atypical basolateral motif of CD147 which comprises a single leucine and is not recognized by RPE cells. This unusual basolateral sorting signal will be useful in unraveling the specialized sorting machinery of RPE cells.

Corresponding author. E-mail: boulan{at}mail.med.cornell.edu




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. J. Belton Jr., L. Chen, F. S. Mesquita, and R. A. Nowak
Basigin-2 Is a Cell Surface Receptor for Soluble Basigin Ligand
J. Biol. Chem., June 27, 2008; 283(26): 17805 - 17814.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Schreiner, M. Ruonala, V. Jakob, J. Suthaus, E. Boles, F. Wouters, and A. Starzinski-Powitz
Junction Protein Shrew-1 Influences Cell Invasion and Interacts with Invasion-promoting Protein CD147
Mol. Biol. Cell, April 1, 2007; 18(4): 1272 - 1281.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
L. Chen, M. Nakai, R. J Belton Jr, and R. A Nowak
Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development
Reproduction, February 1, 2007; 133(2): 405 - 414.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Gravotta, A. Deora, E. Perret, C. Oyanadel, A. Soza, R. Schreiner, A. Gonzalez, and E. Rodriguez-Boulan
AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells
PNAS, January 30, 2007; 104(5): 1564 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Maminishkis, S. Chen, S. Jalickee, T. Banzon, G. Shi, F. E. Wang, T. Ehalt, J. A. Hammer, and S. S. Miller
Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3612 - 3624.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Jakob, A. Schreiner, R. Tikkanen, and A. Starzinski-Powitz
Targeting of Transmembrane Protein Shrew-1 to Adherens Junctions Is Controlled by Cytoplasmic Sorting Motifs
Mol. Biol. Cell, August 1, 2006; 17(8): 3397 - 3408.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Grati, N. Aggarwal, E. E. Strehler, and R. J. Wenthold
Molecular determinants for differential membrane trafficking of PMCA1 and PMCA2 in mammalian hair cells
J. Cell Sci., July 15, 2006; 119(14): 2995 - 3007.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. A. Deora, N. Philp, J. Hu, D. Bok, and E. Rodriguez-Boulan
Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia
PNAS, November 8, 2005; 102(45): 16245 - 16250.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
F. de Mattia, P. J.M. Savelkoul, E.-J. Kamsteeg, I. B.M. Konings, P. van der Sluijs, R. Mallmann, A. Oksche, and P. M.T. Deen
Lack of Arginine Vasopressin-Induced Phosphorylation of Aquaporin-2 Mutant AQP2-R254L Explains Dominant Nephrogenic Diabetes Insipidus
J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2872 - 2880.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. Pena-Munzenmayer, M. Catalan, I. Cornejo, C. D. Figueroa, J. E. Melvin, M. I. Niemeyer, L. P. Cid, and F. V. Sepulveda
Basolateral localization of native ClC-2 chloride channels in absorptive intestinal epithelial cells and basolateral sorting encoded by a CBS-2 domain di-leucine motif
J. Cell Sci., September 15, 2005; 118(18): 4243 - 4252.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Pushkarsky, V. Yurchenko, C. Vanpouille, B. Brichacek, I. Vaisman, S. Hatakeyama, K. I. Nakayama, B. Sherry, and M. I. Bukrinsky
Cell Surface Expression of CD147/EMMPRIN Is Regulated by Cyclophilin 60
J. Biol. Chem., July 29, 2005; 280(30): 27866 - 27871.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. A. Iverson, D. Fox III, L. S. Nadler, R. E. Klevit, and N. M. Nathanson
Identification and Structural Determination of the M3 Muscarinic Acetylcholine Receptor Basolateral Sorting Signal
J. Biol. Chem., July 1, 2005; 280(26): 24568 - 24575.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. E. Newton, Z. Wu, and N. E. Simister
Characterization of basolateral-targeting signals in the neonatal Fc receptor
J. Cell Sci., June 1, 2005; 118(11): 2461 - 2469.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.