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MBC in Press, published online ahead of print July 28, 2004
Mol. Biol. Cell 10.1091/mbc.E04-02-0104

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Submitted on February 5, 2004
Revised on June 28, 2004
Accepted on July 16, 2004

A Ras-like GTPase Is Involved in Hyphal Growth Guidance in the Filamentous Fungus Ashbya gossypii

Yasmina Bauer*{dagger}, Philipp Knechtle*, Jürgen Wendland*{ddagger}, Hanspeter Helfer*, and Peter Philippsen*{sect}

*Biozentrum, University of Basel, CH-4056 Basel, Switzerland; {ddagger}Hans-Knoll Institute and Friedrich-Schiller Universität, D-07745 Jena, Germany

Monitoring Editor: Trisha Davis

Characteristic features of morphogenesis in filamentous fungi are sustained polar growth at tips of hyphae and frequent initiation of novel growth sites (branches) along the extending hyphae. We have begun to study regulation of this process on the molecular level using the model fungus Ashbya gossypii. We found that the A. gossypii Ras-like GTPase Rsr1p/Bud1p localizes to the tip region and that it is involved in apical polarization of the actin cytoskeleton, a determinant of growth direction. In the absence of RSR1/BUD1 hyphal growth was severely slowed down due to frequent phases of pausing of growth at the hyphal tip. During pausing events a hyphal tip marker, encoded by the polarisome component AgSPA2, disappeared from the tip as was shown by in vivo time-lapse fluorescence microscopy of GFP-labeled AgSpa2p. Reoccurrence of AgSpa2p was required for the resumption of hyphal growth. In the Agrsr1{Delta}/bud1{Delta} deletion mutant resumption of growth occurred at the hyphal tip in a frequently uncoordinated manner to the previous axis of polarity. Additionally, hyphal filaments in the mutant developed aberrant branching sites by mislocalizing AgSpa2p thus distorting hyphal morphology. These results define AgRsr1p/Bud1p as a key regulator of hyphal growth guidance.

{dagger}Present address: Actelion Pharmaceuticals Ltd, Gewerbestrasse 16, CH-4123 Allschwil, Switzerland

{sect}Corresponding author. E-mail: peter.philippsen{at}unibas.ch




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