Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 2, 2005
Mol. Biol. Cell 10.1091/mbc.E04-03-0216

A more recent version of this article appeared on April 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-03-0216v1
16/4/1696    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakamura, N.
Right arrow Articles by Hirose, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakamura, N.
Right arrow Articles by Hirose, S.

Submitted on March 15, 2004
Revised on January 6, 2005
Accepted on January 21, 2005

MARCH-II Is a Syntaxin-6-binding Protein Involved in the Endosomal Trafficking

Nobuhiro Nakamura, Hidekazu Fukuda, Akira Kato, and Shigehisa Hirose

Department of Biological Sciences, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan

Monitoring Editor: Jean Gruenberg

MARCH (membrane-associated RING-CH) is a recently identified member of the mammalian E3 ubiquitin ligase family, some members of which down-regulate the expression of immune recognition molecules. Here we have identified MARCH-II, which is ubiquitously expressed and localized to endosomal vesicles and the plasma membrane. Immunoprecipitation and in vitro binding studies established that MARCH-II directly associates with syntaxin 6. Overexpression of MARCH-II resulted in redistribution of syntaxin 6 as well as some syntaxin-6-interacting soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) into the MARCH-II-positive vesicles. In addition, the retrograde transport of TGN38 and a chimeric version of furin to trans-Golgi network (TGN) was perturbed--without affecting the endocytic degradative and biosynthetic secretory pathways--similar to effects caused by a syntaxin 6 mutant lacking the transmembrane domain. MARCH-II overexpression markedly reduced the cell-surface expression of transferrin (Tf) receptor (TfR) and Tf uptake, and interfered with delivery of internalized Tf to perinuclear recycling endosomes. Depletion of MARCH-II by small interfering RNA (siRNA) perturbed the TGN localization of syntaxin 6 and TGN38/46. MARCH-II is thus likely a regulator of trafficking between the TGN and endosomes, which is a novel function for the MARCH family.

Address correspondence to: Shigehisa Hirose (shirose{at}bio.titech.ac.jp)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
N. Nakamura and S. Hirose
Regulation of Mitochondrial Morphology by USP30, a Deubiquitinating Enzyme Present in the Mitochondrial Outer Membrane
Mol. Biol. Cell, May 1, 2008; 19(5): 1903 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
T. Tateno, H. Izumiyama, M. Doi, T. Yoshimoto, M. Shichiri, N. Inoshita, K. Oyama, S. Yamada, and Y. Hirata
Differential gene expression in ACTH -secreting and non-functioning pituitary tumors
Eur. J. Endocrinol., December 1, 2007; 157(6): 717 - 724.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Z. Z. Lieu, M. C. Derby, R. D. Teasdale, C. Hart, P. Gunn, and P. A. Gleeson
The Golgin GCC88 Is Required for Efficient Retrograde Transport of Cargo from the Early Endosomes to the Trans-Golgi Network
Mol. Biol. Cell, December 1, 2007; 18(12): 4979 - 4991.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Morokuma, N. Nakamura, A. Kato, M. Notoya, Y. Yamamoto, Y. Sakai, H. Fukuda, S. Yamashina, Y. Hirata, and S. Hirose
MARCH-XI, a Novel Transmembrane Ubiquitin Ligase Implicated in Ubiquitin-dependent Protein Sorting in Developing Spermatids
J. Biol. Chem., August 24, 2007; 282(34): 24806 - 24815.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Mansouri, J. Douglas, P. P. Rose, K. Gouveia, G. Thomas, R. E. Means, A. V. Moses, and K. Fruh
Kaposi sarcoma herpesvirus K5 removes CD31/PECAM from endothelial cells
Blood, September 15, 2006; 108(6): 1932 - 1940.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Fukuzawa and S. Hirose
Multiple Processing of Ig-Hepta/GPR116, a G Protein-Coupled Receptor with Immunoglobulin (Ig)-Like Repeats, and Generation of EGF2-Like Fragment
J. Biochem., September 1, 2006; 140(3): 445 - 452.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Dumin, I. Bendikov, V. N. Foltyn, Y. Misumi, Y. Ikehara, E. Kartvelishvily, and H. Wolosker
Modulation of D-Serine Levels via Ubiquitin-dependent Proteasomal Degradation of Serine Racemase
J. Biol. Chem., July 21, 2006; 281(29): 20291 - 20302.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. D. Pooley, S. Reddy, V. Soukoulis, J. T. Roland, J. R. Goldenring, and D. M. Bader
CytLEK1 Is a Regulator of Plasma Membrane Recycling through Its Interaction with SNAP-25
Mol. Biol. Cell, July 1, 2006; 17(7): 3176 - 3186.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
H. Fukuda, N. Nakamura, and S. Hirose
MARCH-III Is a Novel Component of Endosomes with Properties Similar to Those of MARCH-II
J. Biochem., January 1, 2006; 139(1): 137 - 145.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.