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MBC in Press, published online ahead of print May 14, 2004
Mol. Biol. Cell 10.1091/mbc.E04-03-0218

A more recent version of this article appeared on July 1, 2004
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Submitted on March 15, 2004
Revised on April 16, 2004
Accepted on May 3, 2004

Rho kinase regulates the intracellular micromechanical response of adherent cells to Rho activation

Thomas P. Kole{dagger}, Yiider Tseng{dagger}*, Lawrence Huang{ddagger}, Joseph L. Katz{dagger}, and Denis Wirtz{dagger}{sect}*

{dagger}Department of Chemical and Biomolecular Engineering, The Johns Hopkins University; {ddagger}Department of Biomedical Engineering, The Johns Hopkins University; {sect}Graduate Program in Molecular Biophysics, The Johns Hopkins University, 3400 N. Charles St., Baltimore, Maryland 21218, USA

Monitoring Editor: Paul Matsudaira

Local sol-gel transitions of the cytoskeleton modulate cell shape changes, which are required for essential cellular functions, including motility and adhesion. In vitro studies using purified cytoskeletal proteins have suggested molecular mechanisms of regulation of cytoskeleton mechanics; however, the mechanical behavior of living cells and the signaling pathways by which it is regulated remains largely unknown. To address this issue, we used a nanoscale sensing method, intracellular microrheology, to examine the mechanical response of the cell to activation of the small GTPase Rho. We observe that the cytoplasmic stiffness and viscosity of serum-starved Swiss 3T3 cells transiently and locally enhances upon treatment with lysophosphatidic acid (LPA), and this mechanical behavior follows a similar trend as Rho activity. Furthermore, the time-dependent activation of Rho decreases the degree of microheterogeneity of the cytoplasm. Our results reveal fundamental differences between intracellular elasticity and cellular tension, and suggest a critical role for Rho kinase in the regulation of intracellular mechanics.

*Corresponding author. E-mail address: yiider{at}jhu.edu

*Corresponding author. E-mail address: wirtz{at}jhu.edu




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