![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on March 1, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on April 28, 2004
Revised on December 2, 2004
Accepted on December 3, 2004
*Institut de Biologie Structurale J-P Ebel (CEA-CNRS-UJF), 38027 Grenoble Cedex 1, France; Laboratoire du Cytosquelette, Inserm U366, DRDC/CS, CEA/Grenoble, 38054 Grenoble Cedex 9, France; Institute for Cancer Research, The Fox Chase Cancer Center, Philadelphia, PA 19111
Monitoring Editor: Ted Salmon
The temporal and spatial regulation of cytokinesis requires an interaction between the anaphase mitotic spindle and the cell cortex. However, the relative roles of the spindle asters or the central spindle bundle are not clear in mammalian cells. The central spindle normally serves as a platform to localize key regulators of cell cleavage, including passenger proteins. Using time-lapse and immunofluorescence analysis, we have addressed the consequences of eliminating the central spindle by ablation of PRC1, a microtubule bundling protein that is critical to the formation of the central spindle. Without a central spindle, the asters guide the equatorial cortical accumulation of anillin and actin, and of the passenger proteins, which organize into a subcortical ring in anaphase. Furrowing goes to completion, but abscission to create two daughter cells fails. We conclude the central spindle bundle is required for abscission, but not for furrowing in mammalian cells.
Present address: Dept. of Cell Biology and Neurosciences, Italian National Institute of Health (ISS), Rome 00161, Italy.
||Corresponding author.
E-mail: margolis{at}ibs.fr
This article has been cited by other articles:
|
|
 |
|
  M. Kanada, A. Nagasaki, and T. Q.P. Uyeda Novel Functions of Ect2 in Polar Lamellipodia Formation and Polarity Maintenance during "Contractile Ring-Independent" Cytokinesis in Adherent Cells Mol. Biol. Cell, January 1, 2008; 19(1): 8 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pavicic-Kaltenbrunner, M. Mishima, and M. Glotzer Cooperative Assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to Form the Centralspindlin Complex Mol. Biol. Cell, December 1, 2007; 18(12): 4992 - 5003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kanehira, T. Katagiri, A. Shimo, R. Takata, T. Shuin, T. Miki, T. Fujioka, and Y. Nakamura Oncogenic Role of MPHOSPH1, a Cancer-Testis Antigen Specific to Human Bladder Cancer Cancer Res., April 1, 2007; 67(7): 3276 - 3285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-m. Zhao, A. Seki, and G. Fang Cep55, a Microtubule-bundling Protein, Associates with Centralspindlin to Control the Midbody Integrity and Cell Abscission during Cytokinesis Mol. Biol. Cell, September 1, 2006; 17(9): 3881 - 3896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Chalamalasetty, S. Hummer, E. A. Nigg, and H. H. W. Sillje Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission J. Cell Sci., July 15, 2006; 119(14): 3008 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhu, E. Lau, R. Schwarzenbacher, E. Bossy-Wetzel, and W. Jiang Spatiotemporal control of spindle midzone formation by PRC1 in human cells PNAS, April 18, 2006; 103(16): 6196 - 6201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Shannon, J. C. Canman, C. B. Moree, J. S. Tirnauer, and E. D. Salmon Taxol-stabilized Microtubules Can Position the Cytokinetic Furrow in Mammalian Cells Mol. Biol. Cell, September 1, 2005; 16(9): 4423 - 4436. [Abstract] [Full Text] [PDF]
|
|
