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MBC in Press, published online ahead of print December 1, 2004
Mol. Biol. Cell 10.1091/mbc.E04-05-0400

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Submitted on May 14, 2004
Accepted on November 17, 2004

An Internal Motor Kinesin Is Associated with the Golgi Apparatus and Plays a Role in Trichome Morphogenesis in Arabidopsis

Ling Lu, Yuh-Ru Julie Lee, Ruiqin Pan, Julin N. Maloof, and Bo Liu*

Section of Plant Biology, University of California, Davis, CA 95616

Monitoring Editor: Tim Stearns

Members of the kinesin superfamily are microtubule-based motor proteins that transport molecules/organelles along microtubules. We have identified similar internal motor kinesins, Kinesin-13A from the cotton Gossypium hirsutum and Arabidopsis thaliana. Their motor domains share high degree of similarity to those of internal motor kinesins of animals and protists in the MCAK/Kinesin13 subfamily. However, no significant sequence similarities were detected in sequences outside the motor domain. In Arabidopsis plants carrying the T-DNA knockout kinesin-13a-1 and kinesin-13a-2 mutations at the Kinesin-13A locus, more than 70% leaf trichomes had four branches whereas wild-type ones had three. Immunofluorescent results showed that AtKinesin-13A and GhKinesin-13A localized to entire Golgi stacks. In both wild-type and kinesin-13a mutant cells, the Golgi stacks were frequently associated with microtubules and with actin microfilaments. Aggregation/clustering of Golgi stacks was often observed in the kinesin-13a mutant trichomes and other epidermal cells. This suggested that the distribution of the Golgi apparatus in cell cortex might require microtubules and Kinesin-13A, and such a motile activity could play a regulatory role in trichome morphogenesis. Our results also indicate that plant kinesins in the MCAK/Kinesin-13 subfamily have evolved to take on different tasks than their animal counterparts.

*Corresponding author. E-mail: bliu{at}ucdavis.edu




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