Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 22, 2004
Mol. Biol. Cell 10.1091/mbc.E04-05-0440

A more recent version of this article appeared on December 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-05-0440v1
15/12/5603    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mitchison, T. J.
Right arrow Articles by Kapoor, T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mitchison, T. J.
Right arrow Articles by Kapoor, T. M.

Submitted on May 31, 2004
Revised on September 2, 2004
Accepted on September 3, 2004

Bipolarization and Poleward Flux Correlate during Xenopus Extract Spindle Assembly

T. J. Mitchison,*{dagger}{ddagger}{sect} P. Maddox,*{ddagger}|| A. Groen,*{dagger}{ddagger} L. Cameron,*{ddagger}|| Z. Perlman,*{dagger} R. Ohi,*{dagger} A. Desai,*¶ E. D. Salmon,*|| and T. M. Kapoor*#

*Cell Division Group, Marine Biological Laboratory, Woods Hole, MA 02543; {dagger}Department of Systems Biology, Harvard Medical School, Boston, MA 02115; ||Department of Biology, University of North Carolina, Chapel Hill, NC 27599; #Rockefeller University, New York, NY 10021; Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093

Monitoring Editor: Tim Stearns

We investigated the mechanism by which meiotic spindles become bipolar, and the correlation between bipolarity and poleward flux, using Xenopus egg extracts. By speckle microscopy and computational alignment, we find that monopolar sperm asters do not show evidence for flux, partially contradicting previous work. We account for the discrepancy by describing spontaneous bipolarization of sperm asters that was missed previously. During spontaneous bipolarization, onset of flux correlated with onset of bipolarity, implying that antiparallel microtubule organization may be required for flux. Using a probe for TPX2 in addition to tubulin, we describe two pathways that lead to spontaneous bipolarization, new pole assembly near chromatin, and pole splitting. By inhibiting the Ran pathway with excess importin-alpha, we establish a role for chromatin-derived, antiparallel overlap bundles in generating the sliding force for flux, and we examine these bundles by EM. Our results highlight the importance of two processes, chromatin-initiated microtubule nucleation, and sliding forces generated between antiparallel microtubules, in self-organization of spindle bipolarity and poleward flux.

{ddagger}These authors contributed equally to the experimental work.

{sect}Corresponding author. E-mail: timothy_mitchison{at}hms.harvard.edu




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. C. Groen, D. Needleman, C. Brangwynne, C. Gradinaru, B. Fowler, R. Mazitschek, and T. J. Mitchison
A novel small-molecule inhibitor reveals a possible role of kinesin-5 in anastral spindle-pole assembly
J. Cell Sci., July 15, 2008; 121(14): 2293 - 2300.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. D. Blower, E. Feric, K. Weis, and R. Heald
Genome-wide analysis demonstrates conserved localization of messenger RNAs to mitotic microtubules
J. Cell Biol., December 31, 2007; 179(7): 1365 - 1373.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
U. Eichenlaub-Ritter, U. Winterscheidt, E. Vogt, Y. Shen, H.-R. Tinneberg, and R. Sorensen
2-Methoxyestradiol Induces Spindle Aberrations, Chromosome Congression Failure, and Nondisjunction in Mouse Oocytes
Biol Reprod, May 1, 2007; 76(5): 784 - 793.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. C. Ems-McClung, K. M. Hertzer, X. Zhang, M. W. Miller, and C. E. Walczak
The Interplay of the N- and C-Terminal Domains of MCAK Control Microtubule Depolymerization Activity and Spindle Assembly
Mol. Biol. Cell, January 1, 2007; 18(1): 282 - 294.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
K. S. Burbank, A. C. Groen, Z. E. Perlman, D. S. Fisher, and T. J. Mitchison
A new method reveals microtubule minus ends throughout the meiotic spindle
J. Cell Biol., November 6, 2006; 175(3): 369 - 375.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. V. Orjalo, A. Arnaoutov, Z. Shen, Y. Boyarchuk, S. G. Zeitlin, B. Fontoura, S. Briggs, M. Dasso, and D. J. Forbes
The Nup107-160 Nucleoporin Complex Is Required for Correct Bipolar Spindle Assembly
Mol. Biol. Cell, September 1, 2006; 17(9): 3806 - 3818.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
L. A. Cameron, G. Yang, D. Cimini, J. C. Canman, O. Kisurina-Evgenieva, A. Khodjakov, G. Danuser, and E.D. Salmon
Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells
J. Cell Biol., April 24, 2006; 173(2): 173 - 179.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. C. Rogers, S. L. Rogers, and D. J. Sharp
Spindle microtubules in flux
J. Cell Sci., March 15, 2005; 118(6): 1105 - 1116.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. T. Miyamoto, Z. E. Perlman, K. S. Burbank, A. C. Groen, and T. J. Mitchison
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles
J. Cell Biol., December 6, 2004; 167(5): 813 - 818.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2004 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.