Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print January 12, 2005
Mol. Biol. Cell 10.1091/mbc.E04-07-0612

A more recent version of this article appeared on March 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-07-0612v1
16/3/1469    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Messina, G.
Right arrow Articles by Grossi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Messina, G.
Right arrow Articles by Grossi, M.

Submitted on July 21, 2004
Revised on December 2, 2004
Accepted on December 23, 2004

p27Kip1 Acts Downstream of N-Cadherin-mediated Cell Adhesion to Promote Myogenesis beyond Cell Cycle Regulation

Graziella Messina, Cristiana Blasi, Severina Anna La Rocca,* Monica Pompili, Attilio Calconi, and Milena Grossi{dagger}

Istituto Pasteur-Fondazione Cenci-Bolognetti, Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma "La Sapienza", 00185 Roma, Italy

Monitoring Editor: Richard Assoian

It is widely acknowledged that cultured myoblasts can not differentiate at very low density. Here we analyzed the mechanism through which cell density influences myogenic differentiation in vitro. By comparing the behavior of C2C12 myoblasts at opposite cell densities, we found that, when cells are sparse, failure to undergo terminal differentiation is independent from cell cycle control and reflects the lack of p27Kip1 and MyoD in proliferating myoblasts. We show that inhibition of p27Kip1 expression impairs C2C12 cells differentiation at high density, while exogenous p27Kip1 allows low-density cultured C2C12 cells to enter the differentiative program by regulating MyoD levels in undifferentiated myoblasts. We also demonstrate that the early induction of p27Kip1 is a critical step of the N-cadherin-dependent signaling involved in myogenesis. Overall, our data support an active role of p27Kip1 in the decision of myoblasts to commit to terminal differentiation, distinct from the regulation of cell proliferation, and identify a pathway that, reasonably, operates in vivo during myogenesis and might be part of the phenomenon known as "community effect".

*Present address: Department of Immunology and Pathology, Institute of Animal Health, Pirbright Surrey, GU24 ONF United Kingdom.

{dagger}Corresponding author. E-mail: milena.grossi{at}uniroma1.it




This article has been cited by other articles:


Home page
 Cancer Res.Home page
J. Kucharczak, S. Charrasse, F. Comunale, J. Zappulla, B. Robert, I. Teulon-Navarro, A. Pelegrin, and C. Gauthier-Rouviere
R-Cadherin Expression Inhibits Myogenesis and Induces Myoblast Transformation via Rac1 GTPase
Cancer Res., August 15, 2008; 68(16): 6559 - 6568.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Theard, M. A. Raspe, D. Kalicharan, D. Hoekstra, and S. C.D. van IJzendoorn
Formation of E-Cadherin/{beta}-Catenin-based Adherens Junctions in Hepatocytes Requires Serine-10 in p27(Kip1)
Mol. Biol. Cell, April 1, 2008; 19(4): 1605 - 1613.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
D. Pajalunga, A. Mazzola, A. M. Salzano, M. G. Biferi, G. De Luca, and M. Crescenzi
Critical requirement for cell cycle inhibitors in sustaining nonproliferative states
J. Cell Biol., March 12, 2007; 176(6): 807 - 818.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
R. Sarig, Z. Baruchi, O. Fuchs, U. Nudel, and D. Yaffe
Regeneration and Transdifferentiation Potential of Muscle-Derived Stem Cells Propagated as Myospheres
Stem Cells, July 1, 2006; 24(7): 1769 - 1778.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. Lees, C. R. Rathbone, and F. W. Booth
Age-associated decrease in muscle precursor cell differentiation
Am J Physiol Cell Physiol, February 1, 2006; 290(2): C609 - C615.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.