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A more recent version of this article appeared on August 1, 2005
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Submitted on December 14, 2004
Revised on May 11, 2005
Accepted on May 19, 2005
-Tubulin, and NUDF/LIS1 in Aspergillus nidulans
*Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814; Department of Molecular Genetics, Ohio State University, Columbus, OH 43210; Richard Montgomery High School, Rockville, MD 20852
Monitoring Editor: Yixian Zheng
In Aspergillus nidulans, cytoplasmic dynein and NUDF/LIS1 are found at the spindle poles during mitosis, but they appear to be targeted to this location via different mechanisms. The spindle-pole localization of cytoplasmic dynein requires the function of the anaphase-promoting complex (APC), while that of NUDF does not. Moreover, while NUDF’s localization to the spindle poles does not require a fully functional dynein motor, the function of NUDF is important for cytoplasmic dynein’s targeting to the spindle poles. Interestingly, a 
-tubulin mutation, mipAR63, nearly eliminates the localization of cytoplasmic dynein to the spindle poles, but has no apparent effect on NUDF’s spindle-pole localization. Live cell analysis of the mipAR63 mutant revealed a defect in chromosome separation accompanied by unscheduled spindle elongation before the completion of anaphase A, suggesting that -tubulin may recruit regulatory proteins to the spindle poles for mitotic progression. In A. nidulans, dynein is not apparently required for mitotic progression. In the presence of a low amount of benomyl, a microtubule depolymerizing agent, however, a dynein mutant diploid strain exhibits a more pronounced chromosome-loss phenotype than the control, indicating that cytoplasmic dynein plays a role in chromosome segregation.
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