Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print May 31, 2005
Mol. Biol. Cell 10.1091/mbc.E04-12-1110

A more recent version of this article appeared on July 1, 2005 Originally published as MBC in Press, 10.1091/mbc.E04-12-1110 on May 11, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E04-12-1110v1
E04-12-1110v2
16/7/3176    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morales-Mulia, S.
Right arrow Articles by Scholey, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morales-Mulia, S.
Right arrow Articles by Scholey, J. M.

Submitted on December 23, 2004
Accepted on April 27, 2005

Spindle Pole Organization in Drosophila S2 Cells by Dynein, Abnormal Spindle Protein (Asp), and KLP10A

Sandra Morales-Mulia and Jonathan M. Scholey

Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California-Davis, Davis, CA 95616

Monitoring Editor: Yixian Zheng

Dynein is a critical mitotic motor whose inhibition causes defects in spindle pole organization and separation, chromosome congression or segregation, and anaphase spindle elongation, but results differ in different systems. We evaluated the functions of the dynein-dynactin complex using RNAi-mediated depletion of distinct subunits in Drosophila S2 cells. We observed a striking detachment of centrosomes from spindles, an increase in spindle length and a loss of spindle pole focus. RNAi-depletion of Ncd, another minus-end motor, produced disorganized spindles consisting of multiple disconnected minispindles, a different phenotype consistent with distinct pathways of spindle pole organization. Two candidate dynein-dependent spindle pole organizers were also investigated. RNAi-depletion of the abnormal spindle protein, Asp, which localizes to focused poles of control spindles produced a severe loss of spindle pole focus whereas depletion of the pole-associated MT depolymerase KLP10A increased spindle microtubule density. Depletion of either protein produced long spindles. Following RNAi-depletion of dynein-dynactin we observed subtle but significant mislocalization of KLP10A and Asp, suggesting that dynein-dynactin, Asp and KLP10A have complex interdependent functions in spindle pole focusing and centrosome attachment. These results extend recent findings from Xenopus extracts to Drosophila cultured cells and suggest common pathways in spindle pole organization and length determination.

Address correspondence to: Jonathan M. Scholey (jmscholey{at}ucdavis.edu)




This article has been cited by other articles:


Home page
 Genes Dev.Home page
M. Kwon, S. A. Godinho, N. S. Chandhok, N. J. Ganem, A. Azioune, M. Thery, and D. Pellman
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
Genes & Dev., August 15, 2008; 22(16): 2189 - 2203.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Trammell, N. M. Mahoney, D. A. Agard, and R. D. Vale
Mob4 plays a role in spindle focusing in Drosophila S2 cells
J. Cell Sci., April 15, 2008; 121(8): 1284 - 1292.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Oladipo, A. Cowan, and V. Rodionov
Microtubule Motor Ncd Induces Sliding of Microtubules In Vivo
Mol. Biol. Cell, September 1, 2007; 18(9): 3601 - 3606.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. L. Grishchuk, I. S. Spiridonov, and J. R. McIntosh
Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end-directed, Kinesin-like Protein
Mol. Biol. Cell, June 1, 2007; 18(6): 2216 - 2225.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
D. Zhang, G. C. Rogers, D. W. Buster, and D. J. Sharp
Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes
J. Cell Biol., April 23, 2007; 177(2): 231 - 242.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
G. Goshima, R. Wollman, S. S. Goodwin, N. Zhang, J. M. Scholey, R. D. Vale, and N. Stuurman
Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells
Science, April 20, 2007; 316(5823): 417 - 421.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
H. Kim, S.-C. Ling, G. C. Rogers, C. Kural, P. R. Selvin, S. L. Rogers, and V. I. Gelfand
Microtubule binding by dynactin is required for microtubule organization but not cargo transport
J. Cell Biol., February 26, 2007; 176(5): 641 - 651.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J.-G. Delcros, C. Prigent, and R. Giet
Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells
J. Cell Sci., November 1, 2006; 119(21): 4431 - 4441.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Zhou, W. Zimmerman, X. Liu, and R. L. Erikson
A mammalian NudC-like protein essential for dynein stability and cell viability
PNAS, June 13, 2006; 103(24): 9039 - 9044.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
G. Goshima, F. Nedelec, and R. D. Vale
Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins
J. Cell Biol., October 24, 2005; 171(2): 229 - 240.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.