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A more recent version of this article appeared on July 1, 2005
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Submitted on January 4, 2005
Revised on March 29, 2005
Accepted on April 22, 2005
*Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, 28006 Madrid, Spain; Instituto de Farmacología y Toxicología, CSIC-UCM, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain; Vita-Salute San Raffaele University School of Medicine, 20132 Milano, Italy
Monitoring Editor: Mark Ginsberg
Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAMs-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.
These authors contributed equally to this work.
Address correspondence to:
Francisco Sánchez-Madrid (fsanchez.hlpr{at}salud.madrid.org)
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