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MBC in Press, published online ahead of print August 17, 2005
Mol. Biol. Cell 10.1091/mbc.E05-01-0007

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Submitted on January 6, 2005
Revised on August 8, 2005
Accepted on August 9, 2005

Cell-Matrix Entanglement and Mechanical Anchorage of Fibroblasts in Three-dimensional Collagen Matrices

Hongmei Jiang and Frederick Grinnell

Department of Cell Biology, University of Texas Southwestern Medical School, Dallas, TX 75390-9039

Monitoring Editor: Jean Schwarzbauer

Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips, phagocytosis of fibronectin-coated beads by fibroblasts in collagen matrices was found to be reduced. This decrease could not be explained by integrin reorganization, tight binding of FN beads to the collagen matrix, or differences in overall bead binding to the cells. Rather, entanglement of cellular dendritic extensions with collagen fibrils appeared to interfere with the ability of the extensions to interact with the beads. Moreover, once these extensions became entangled in the matrix, cells developed an integrin-independent component of adhesion. We suggest that cell-matrix entanglement represents a novel mechanism of cell anchorage that uniquely depends on the three-dimensional character of the matrix.

Address correspondence to: Frederick Grinnell (frederick.grinnell{at}utsouthwestern.edu)




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