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MBC in Press, published online ahead of print April 13, 2005
Mol. Biol. Cell 10.1091/mbc.E05-02-0103

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Submitted on February 9, 2005
Revised on April 4, 2005
Accepted on April 5, 2005

Dynein Light Chain 1 Regulates Dynamin Mediated F-Actin Assembly during Sperm Individualization in Drosophila

Anindya Ghosh-Roy,* Bela S. Desai,* and Krishanu Ray

Tata Institute of Fundamental Research, Mumbai 400 005, India

Monitoring Editor: Suzanne Pfeffer

Toward the end of spermiogenesis, spermatid nuclei are compacted and the clonally related spermatids individualize to become mature and active sperm. Studies in Drosophila showed that caudal end directed movement of a microfilament rich structure, called investment cone, expels the cytoplasmic contents of individual spermatids. F-actin dynamics plays an important role in this process. Here we report that the dynein light chain 1 (DLC1) of Drosophila is involved in two separate cellular processes during sperm individualization. It is enriched around spermatid nuclei during postelongation stages and plays an important role in the dynein-dynactin dependent rostral retention of the nuclei during this period. In addition, DDLC1 colocalizes with dynamin along investment cones and regulates F-actin assembly at this organelle by retaining dynamin along the cones. Interestingly, we found that this process does not require the other subunits of cytoplasmic dynein-dynactin complex. Altogether, these observations suggest that DLC1 could independently regulate multiple cellular functions and established a novel role of this protein in F-actin assembly in Drosophila.

*These authors made equal contributions to the manuscript.

Address correspondence to: Krishanu Ray (krishanu{at}tifr.res.in)




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