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MBC in Press, published online ahead of print June 15, 2005
Mol. Biol. Cell 10.1091/mbc.E05-02-0136

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Submitted on February 16, 2005
Revised on June 3, 2005
Accepted on June 7, 2005

Assembly of Urothelial Plaques: Tetraspanin Function in Membrane Protein Trafficking

Chih-Chi Andrew Hu,*{dagger} Feng-Xia Liang,* Ge Zhou,* Liyu Tu,{ddagger} Chih-Hang Anthony Tang,* Jessica Zhou,* Gert Kreibich,{ddagger}{sect} and Tung-Tien Sun*{dagger}{sect}||

*Epithelial Biology Unit, The Ronald O. Perelman Department of Dermatology and Departments of {dagger}Pharmacology, {ddagger}Cell Biology, and ||Urology; and {sect}NYU Cancer Institute, New York University School of Medicine, New York, NY 10016

Monitoring Editor: Jeffrey Brodsky

The apical surface of mammalian urothelium is covered by 16-nm protein particles packed hexagonally to form 2D crystals of asymmetric unit membranes (AUM) that contribute to the remarkable permeability barrier function of the urinary bladder. We have shown previously that bovine AUMs contain four major integral membrane proteins, i.e., uroplakins Ia, Ib, II and IIIa, and that UPIa and Ib (both tetraspanins) form heterodimers with UPII and IIIa, respectively. Using a panel of antibodies recognizing different conformational states of uroplakins, we demonstrate that the UPIa-dependent, furin-mediated cleavage of the prosequence of UPII leads to global conformational changes in mature UPII, and that UPIb also induces conformational changes in its partner UPIIIa. We further demonstrate that tetraspanins CD9, CD81 and CD82 can stabilize their partner protein CD4. These results indicate that tetraspanin uroplakins, and some other tetraspanin proteins, can induce conformational changes leading to the ER-exit, stabilization and cell surface expression of their associated, single-transmembrane-domained partner proteins, and thus can function as ‘maturation-facilitators.’ We propose a model of AUM assembly in which conformational changes in integral membrane proteins induced by uroplakin interactions, differentiation-dependent glycosylation and the removal of the prosequence of UPII play roles in regulating the assembly of uroplakins to form AUM.

Address correspondence to: Tung-Tien Sun (sunt01{at}med.nyu.edu)




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