Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 29, 2005
Mol. Biol. Cell 10.1091/mbc.E05-03-0204

A more recent version of this article appeared on December 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E05-03-0204v1
16/12/5699    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strick, D. J.
Right arrow Articles by Elferink, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strick, D. J.
Right arrow Articles by Elferink, L. A.

Submitted on March 11, 2005
Revised on September 7, 2005
Accepted on September 16, 2005

Rab15 Effector Protein: A Novel Protein for Receptor Recycling from the Endocytic Recycling Compartment

David J. Strick* and Lisa A. Elferink*{dagger}

*Department of Neuroscience and Cell Biology and {dagger}Sealy Center for Cancer Cell Biology, University of Texas Medical Branch, Galveston, TX 77555-1043

Monitoring Editor: Suzanne Pfeffer

Sorting Endosomes and the Endocytic Recycling Compartment are critical intracellular stores for the rapid recycling of internalized membrane receptors to the cell surface in multiple cell types. However, the molecular mechanisms distinguishing fast receptor recycling from Sorting Endosomes, and slow receptor recycling from the Endocytic Recycling Compartment remain poorly understood. We previously reported that Rab15 differentially regulates Transferrin Receptor trafficking through Sorting Endosomes and the Endocytic Recycling Compartment (Zuk and Elferink, 2000), suggesting a role for distinct Rab15-effector interactions at these endocytic compartments. In this study, we identified the novel protein Rab15 Effector Protein (REP15) as a binding partner for Rab15-GTP. REP15 is compartment specific, colocalizing with Rab15 and Rab11 on the Endocytic Recycling Compartment but not with Rab15, Rab4, or EEA1 on Sorting Endosomes. REP15 interacts directly with Rab15-GTP, but not with Rab5 or Rab11. Consistent with its localization, REP15 over expression and siRNA-mediated depletion inhibited Transferrin Receptor recycling from the Endocytic Recycling Compartment, without affecting receptor entry into or recycling from Sorting Endosomes. Our data identify REP15 as a compartment specific protein for receptor recycling from the Endocytic Recycling Compartment, highlighting that the rapid and slow modes of Transferrin Receptor recycling are mechanistically distinct pathways.

Address correspondence to: Lisa A. Elferink (laelferi{at}utmb.edu)




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
D. M. Henderson and S. D. Conner
A Novel AAK1 Splice Variant Functions at Multiple Steps of the Endocytic Pathway
Mol. Biol. Cell, July 1, 2007; 18(7): 2698 - 2706.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.