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MBC in Press, published online ahead of print August 24, 2005
Mol. Biol. Cell 10.1091/mbc.E05-04-0272

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Submitted on April 3, 2005
Revised on August 12, 2005
Accepted on August 16, 2005

Myosin-V, Kinesin-1, and Kinesin-3 Cooperate in Long-Distance Transport in Hyphal Growth of the Fungus Ustilago maydis

Isabel Schuchardt,* Daniela Aßmann,* Eckhard Thines,{dagger} Christian Schuberth,{ddagger} and Gero Steinberg*

*Max-Planck-Institut für Terrestrische Mikrobiologie, Karl-von-Frisch-Straße, D-35043 Marburg, Germany; {dagger}Eckhard Thines, Institut für Biotechnologie und Wirkstoff-Forschung, TU Kaiserslautern, D-67663 Kaiserslautern, Germany; {ddagger}Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

Monitoring Editor: David Drubin

Long-distance transport is crucial for polar growing cells, such as neurons and fungal hyphae. Kinesins and myosins participate in this, but their functional interplay is poorly understood. Here we investigate the role of kinesin motors in hyphal growth of the plant pathogen Ustilago maydis. Although the microtubule plus-ends are directed to the hyphal tip, out of all 10 kinesins analyzed, only conventional kinesin (Kinesin-1), and Unc104/Kif1A-like kinesin (Kinesin-3) were up-regulated in hyphae and are essential for extended hyphal growth. {Delta}kin1 and {Delta}kin3 mutant hyphae grew irregular and remained short, but were still able to grow polarized. No additional phenotype was detected in {Delta}kin1rkin3 double mutants, but polarity was lost in {Delta}myo5rkin1 and {Delta}myo5rkin3 mutant cells, suggesting that kinesins and class V myosin cooperate in hyphal growth. Consistent with such a role in secretion, fusion proteins of GFP and Kinesin-1, Myosin-V and Kinesin-3 accumulate in the apex of hyphae, a region where secretory vesicles cluster to form the fungal Spitzenkörper. Quantitative assays revealed a role of Kin3 in secretion of acid phosphatase, whereas Kin1 was not involved. Our data demonstrate that just two kinesins and at least one myosin support hyphal growth.

Address correspondence to: Gero Steinberg (Gero.Steinberg{at}staff.uni-marburg.de)




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