Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 21, 2005
Mol. Biol. Cell 10.1091/mbc.E05-05-0410

A more recent version of this article appeared on December 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Video
Right arrow All Versions of this Article:
E05-05-0410v1
16/12/5514    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by El Sayegh, T. Y.
Right arrow Articles by Kapus, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by El Sayegh, T. Y.
Right arrow Articles by Kapus, A.

Submitted on May 10, 2005
Revised on August 25, 2005
Accepted on September 13, 2005

Phosphorylation of N-Cadherin-associated Cortactin by Fer Kinase Regulates N-Cadherin Mobility and Intercellular Adhesion Strength

T. Y. El Sayegh,* P. D. Arora,* L. Fan,{dagger} C. A. Laschinger,* P. A. Greer,{ddagger} C. A. McCulloch,* and A. Kapus{dagger}

*CIHR Group in Matrix Dynamics, University of Toronto, Toronto, Ontario M5S 3E2, Canada; {ddagger}Department of Pathology and Molecular Medicine, Division of Cancer Biology and Genetics, Queen’s University Cancer Research Institute, Kingston, Ontario K7L 3N6, Canada; {dagger}Department of Surgery, University of Toronto and St. Michael’s Hospital Research Institute, Toronto, Ontario M5B 1W8, Canada

Monitoring Editor: Richard Assoian

Cortactin regulates the strength of nascent N-cadherin-mediated intercellular adhesions through a tyrosine phosphorylation-dependent mechanism. Currently, the functional significance of cortactin phosphorylation and the kinases responsible for the regulation of adhesion strength are not defined. We show that the nonreceptor tyrosine kinase Fer phosphorylates cadherin-associated cortactin, and that this process is involved in mediating intercellular adhesion strength. In wild-type fibroblasts N-cadherin ligation induced transient phosphorylation of Fer, indicating that junction formation activates Fer kinase. Tyrosine phosphorylation of cortactin after N-cadherin ligation was strongly reduced in fibroblasts expressing only catalytically inactive Fer (D743R), compared with wild-type cells. In wild-type cells, N-cadherin coated bead pull-off assays induced fourfold greater endogenous N-cadherin association than in D743R cells. Fluorescence recovery after photobleaching showed that GFP-N-cadherin mobility at nascent contacts was 50% faster in wild-type than D743R cells. In shear wash-off assays, nascent intercellular adhesion strength was twofold higher in wild-type than D743R cells. Cortactin recruitment to adhesions was independent of Fer kinase activity, but was impacted by N-cadherin ligation-provoked Rac activation. We conclude that N-cadherin ligation induces Rac-dependent cortactin recruitment and Fer-dependent cortactin phosphorylation, which in turn promotes enhanced mobilization and interaction of surface expressed N-cadherin in contacting cells.

Address correspondence to: T. Y. El Sayegh (t.elsayegh{at}utoronto.ca)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. T. Herrera Abreu, P. Castellanos Penton, V. Kwok, E. Vachon, D. Shalloway, L. Vidali, W. Lee, C. A. McCulloch, and G. P. Downey
Tyrosine phosphatase PTP{alpha} regulates focal adhesion remodeling through Rac1 activation
Am J Physiol Cell Physiol, April 1, 2008; 294(4): C931 - C944.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Y. El Sayegh, P. D. Arora, K. Ling, C. Laschinger, P. A. Janmey, R. A. Anderson, and C. A. McCulloch
Phosphatidylinositol-4,5 Bisphosphate Produced by PIP5KI{gamma} Regulates Gelsolin, Actin Assembly, and Adhesion Strength of N-Cadherin Junctions
Mol. Biol. Cell, August 1, 2007; 18(8): 3026 - 3038.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D. Dajnowiec, P. J.B. Sabatini, T. C. Van Rossum, J. T.K. Lam, M. Zhang, A. Kapus, and B. L. Langille
Force-Induced Polarized Mitosis of Endothelial and Smooth Muscle Cells in Arterial Remodeling
Hypertension, July 1, 2007; 50(1): 255 - 260.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. A. Parsons and P. A. Greer
The Fps/Fes kinase regulates the inflammatory response to endotoxin through down-regulation of TLR4, NF-{kappa}B activation, and TNF-{alpha} secretion in macrophages
J. Leukoc. Biol., December 1, 2006; 80(6): 1522 - 1528.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
L. I. Cosen-Binker and A. Kapus
Cortactin: The Gray Eminence of the Cytoskeleton.
Physiology, October 1, 2006; 21(5): 352 - 361.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. M. Gonzalez Jr, J. A. Faralli, J. M. Peters, J. R. Newman, and D. M. Peters
Effect of Heparin II Domain of Fibronectin on Actin Cytoskeleton and Adherens Junctions in Human Trabecular Meshwork Cells.
Invest. Ophthalmol. Vis. Sci., July 1, 2006; 47(7): 2924 - 2931.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
O. Thoumine, M. Lambert, R.-M. Mege, and D. Choquet
Regulation of N-Cadherin Dynamics at Neuronal Contacts by Ligand Binding and Cytoskeletal Coupling
Mol. Biol. Cell, February 1, 2006; 17(2): 862 - 875.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.