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MBC in Press, published online ahead of print September 29, 2005
Mol. Biol. Cell 10.1091/mbc.E05-08-0706

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Submitted on August 2, 2005
Revised on September 13, 2005
Accepted on September 15, 2005

Centromere Positioning and Dynamics in Living Arabidopsis Plants

Yuda Fang and David L. Spector

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724

Monitoring Editor: Joseph Gall

The organization and dynamics of the genome has been shown to influence gene expression in many organisms. Data from mammalian tissue culture cells have provided conflicting conclusions with regard to the extent to which chromatin organization is inherited from mother to daughter nuclei. To gain insight into chromatin organization and dynamics we developed transgenic Arabidopsis lines in which centromeres were tagged with a GFP fusion of the centromere-specific histone H3. Using four-dimensional (4-D) live-cell imaging, we show that Arabidopsis centromeres are constrained at the nuclear periphery during interphase, and the organization of endoreduplicated sister centromeres is cell-type dependent with predominant clustering in leaf epidermal cells and dispersion in root epidermal cells. 4-D tracking of the entire set of centromeres through mitosis, in growing root meristemic cells, demonstrated that global centromere position is not precisely transmitted from the mother cell to daughter cells. These results provide important insight into our understanding of chromatin organization among different cells of a living organism.

Address correspondence to: David L. Spector (spector{at}cshl.edu)




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