Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 29, 2005
Mol. Biol. Cell 10.1091/mbc.E05-08-0756

A more recent version of this article appeared on December 1, 2005
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E05-08-0756v1
16/12/5563    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Colot, H. V.
Right arrow Articles by Dunlap, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Colot, H. V.
Right arrow Articles by Dunlap, J. C.

Submitted on August 15, 2005
Revised on September 18, 2005
Accepted on September 20, 2005

Temperature-modulated Alternative Splicing and Promoter Use in the Circadian Clock Gene frequency

Hildur V. Colot,* Jennifer J. Loros,{dagger} and Jay C. Dunlap*

Departments of *Genetics and {dagger}Biochemistry, Dartmouth Medical School, Hanover, NH 03755

Monitoring Editor: Greg Matera

The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio as a function of temperature (Garceau et al., 1997; Liu et al., 1997) contributes to robust rhythmicity across a range of temperatures. We have found frq expression to be surprisingly complex, despite our earlier prediction of a simple transcription unit based on limited cDNA sequencing. Two distinct environmentally-regulated major promoters drive primary transcripts whose environmentally-influenced alternative splicing gives rise to six different major mRNA species as well as minor forms. Temperature-sensitive alternative splicing determines AUG choice and, as a consequence, the ratio of LFRQ to SFRQ. Four of the six upstream ORFs are spliced out of the vast majority of frq mRNA species. Alternative splice site choice in the 5` UTR and relative use of two major promoters are also influenced by temperature, and the two promoters are differentially regulated by light. Evolutionary comparisons with the Sordariaceae reveal conservation of 5` UTR sequences, as well as significant conservation of the alternative splicing events, supporting their relevance to proper regulation of clock function.

Address correspondence to: Jay C. Dunlap (jay.c.dunlap{at}dartmouth.edu)




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
V. D. Gooch, A. Mehra, L. F. Larrondo, J. Fox, M. Touroutoutoudis, J. J. Loros, and J. C. Dunlap
Fully Codon-Optimized luciferase Uncovers Novel Temperature Characteristics of the Neurospora Clock
Eukaryot. Cell, January 1, 2008; 7(1): 28 - 37.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Yakir, D. Hilman, M. Hassidim, and R. M. Green
CIRCADIAN CLOCK ASSOCIATED1 Transcript Stability and the Entrainment of the Circadian Clock in Arabidopsis
Plant Physiology, November 1, 2007; 145(3): 925 - 932.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Goetz, L. C. Hooper, S. D. Johnson, J. C. M. Rodrigues, A. Vivian-Smith, and A. M. Koltunow
Expression of Aberrant Forms of AUXIN RESPONSE FACTOR8 Stimulates Parthenocarpy in Arabidopsis and Tomato
Plant Physiology, October 1, 2007; 145(2): 351 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. M. Hunt, M. Elvin, S. K. Crosthwaite, and C. Heintzen
The PAS/LOV protein VIVID controls temperature compensation of circadian clock phase and development in Neurospora crassa
Genes & Dev., August 1, 2007; 21(15): 1964 - 1974.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. L. Rukov, M. Irimia, S. Mork, V. K. Lund, J. Vinther, and P. Arctander
High Qualitative and Quantitative Conservation of Alternative Splicing in Caenorhabditis elegans and Caenorhabditis briggsae
Mol. Biol. Evol., April 1, 2007; 24(4): 909 - 917.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
J.C. Dunlap, J.J. Loros, H.V. Colot, A. Mehra, W.J. Belden, M. Shi, C.I. Hong, L.F. Larrondo, C.L. Baker, C.-H. Chen, et al.
A Circadian Clock in Neurospora: How Genes and Proteins Cooperate to Produce a Sustained, Entrainable, and Compensated Biological Oscillator with a Period of about a Day
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 57 - 68.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
E. Garbarino-Pico and C. B. Green
Posttranscriptional Regulation of Mammalian Circadian Clock Output
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 145 - 156.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
J. J. Loros, J. C. Dunlap, L. F. Larrondo, M. Shi, W. J. Belden, V. D. Gooch, C.-H. Chen, C. L. Baker, A. Mehra, H. V. Colot, et al.
Circadian Output, Input, and Intracellular Oscillators: Insights into the Circadian Systems of Single Cells
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 201 - 214.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
W.-F. Chen, K. H. Low, C. Lim, and I. Edery
Thermosensitive Splicing of a Clock Gene and Seasonal Adaptation
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 599 - 606.
[Abstract] [PDF]

Home page
 J Biol RhythmsHome page
M. W. Vitalini, R. M. de Paula, W. D. Park, and D. Bell-Pedersen
The Rhythms of Life: Circadian Output Pathways in Neurospora.
J Biol Rhythms, December 1, 2006; 21(6): 432 - 444.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J. C. Dunlap
Proteins in the Neurospora Circadian Clockworks
J. Biol. Chem., September 29, 2006; 281(39): 28489 - 28493.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
M. S. Sachs and A. P. Geballe
Downstream control of upstream open reading frames
Genes & Dev., April 15, 2006; 20(8): 915 - 921.
[Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2005 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.