![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on February 1, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on August 29, 2005
Revised on October 20, 2005
Accepted on November 10, 2005
Department of Cell Biology, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany
Monitoring Editor: Yixian Zheng
The anchoring of microtubules (MTs) to subcellular structures is critical for cell shape, polarity and motility. In mammalian cells, the centrosome is a prominent MT anchoring structure. A number of proteins, including ninein, p150Glued and EB1, have been implicated in centrosomal MT anchoring, but the process is far from understood. Here we show that CAP350 and FOP (FGFR1 oncogene partner) form a centrosomal complex required for MT anchoring. We show that the C-terminal domain of CAP350 interacts directly with FOP and that both proteins localize to the centrosome throughout the cell cycle. FOP also binds to EB1 and is required for localizing EB1 to the centrosome. Depletion of either CAP350, FOP or EB1 by siRNA causes loss of MT anchoring and profound disorganization of the MT network. These results have implications for the mechanisms underlying MT anchoring at the centrosome and they attribute a key MT anchoring function to two novel centrosomal proteins, CAP350 and FOP.
This article has been cited by other articles:
|
|
 |
|
  J. Azimzadeh, P. Nacry, A. Christodoulidou, S. Drevensek, C. Camilleri, N. Amiour, F. Parcy, M. Pastuglia, and D. Bouchez Arabidopsis TONNEAU1 Proteins Are Essential for Preprophase Band Formation and Interact with Centrin PLANT CELL, August 1, 2008; 20(8): 2146 - 2159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Burakov, O. N. Zhapparova, O. V. Kovalenko, L. A. Zinovkina, E. S. Potekhina, N. A. Shanina, D. G. Weiss, S. A. Kuznetsov, and E. S. Nadezhdina Ste20-related Protein Kinase LOSK (SLK) Controls Microtubule Radial Array in Interphase Mol. Biol. Cell, May 1, 2008; 19(5): 1952 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Graser, Y.-D. Stierhof, and E. A. Nigg Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion J. Cell Sci., December 15, 2007; 120(24): 4321 - 4331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hoppeler-Lebel, C. Celati, G. Bellett, M. M. Mogensen, L. Klein-Hitpass, M. Bornens, and A.-M. Tassin Centrosomal CAP350 protein stabilises microtubules associated with the Golgi complex J. Cell Sci., September 15, 2007; 120(18): 3299 - 3308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Azimzadeh and M. Bornens Structure and duplication of the centrosome J. Cell Sci., July 1, 2007; 120(13): 2139 - 2142. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Van Leene, H. Stals, D. Eeckhout, G. Persiau, E. Van De Slijke, G. Van Isterdael, A. De Clercq, E. Bonnet, K. Laukens, N. Remmerie, et al. A Tandem Affinity Purification-based Technology Platform to Study the Cell Cycle Interactome in Arabidopsis thaliana Mol. Cell. Proteomics, July 1, 2007; 6(7): 1226 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jeong, J. Lee, K. Kim, J. C. Yoo, and K. Rhee Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization J. Cell Sci., June 15, 2007; 120(12): 2106 - 2116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Wood, D. A. Dumesic, D. H. Abbott, and J. F. Strauss III Molecular Abnormalities in Oocytes from Women with Polycystic Ovary Syndrome Revealed by Microarray Analysis J. Clin. Endocrinol. Metab., February 1, 2007; 92(2): 705 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lechler and E. Fuchs Desmoplakin: an unexpected regulator of microtubule organization in the epidermis J. Cell Biol., January 16, 2007; 176(2): 147 - 154. [Abstract] [Full Text] [PDF]
|
|
