![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on May 1, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on October 11, 2005
Revised on February 2, 2006
Accepted on February 8, 2006
*Division of Pharmaceutical Sciences, Laboratory of Genetics, and School of Pharmacy, University of Wisconsin, Madison, WI 53705
Monitoring Editor: Martin Chalfie
Cilia serve as sensory devices in a diversity of organisms and their defects contribute to many human diseases. In primary cilia of kidney cells, the transient receptor potential polycystin (TRPP) channels polycystin-1 (PC-1) and polycystin-2 (PC-2) act as a mechanosensitive channel, with defects resulting in autosomal dominant polycystic kidney disease. In sensory cilia of Caenorhabditis elegans male-specific neurons, the TRPPs LOV-1 and PKD-2 are required for mating behavior. The mechanisms regulating TRPP ciliary localization and function are largely unknown. We identified the regulatory subunit of the serine-theonine casein kinase II (CK2) as a binding partner of LOV-1 and human PC-1. CK2 and the calcineurin phosphatase TAX-6 modulate male mating behavior and PKD-2 ciliary localization. The phospho-defective mutant PKD-2S534A localizes to cilia while a phospho-mimetic PKD-2S534D mutant is largely absent from cilia. Calcineurin is required for PKD-2 ciliary localization, but is not essential for ciliary gene expression, ciliogenesis, or localization of cilium structural components. This unanticipated function of calcineurin may be important for regulating ciliary protein localization. A dynamic phosphorylation-dephosphorylation cycle may represent a mechanism for modulating TRPP activity, cellular sensation, and ciliary protein localization.
This article has been cited by other articles:
|
|
 |
|
  T. Bachhuber, J. Almaca, F. Aldehni, A. Mehta, M. D. Amaral, R. Schreiber, and K. Kunzelmann Regulation of the Epithelial Na+ Channel by the Protein Kinase CK2 J. Biol. Chem., May 9, 2008; 283(19): 13225 - 13232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. F. Berbari, A. D. Johnson, J. S. Lewis, C. C. Askwith, and K. Mykytyn Identification of Ciliary Localization Sequences within the Third Intracellular Loop of G Protein-coupled Receptors Mol. Biol. Cell, April 1, 2008; 19(4): 1540 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sohara, Y. Luo, J. Zhang, D. K. Manning, D. R. Beier, and J. Zhou Nek8 Regulates the Expression and Localization of Polycystin-1 and Polycystin-2 J. Am. Soc. Nephrol., March 1, 2008; 19(3): 469 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Gradilone, A. I. Masyuk, P. L. Splinter, J. M. Banales, B. Q. Huang, P. S. Tietz, T. V. Masyuk, and N. F. LaRusso Cholangiocyte cilia express TRPV4 and detect changes in luminal tonicity inducing bicarbonate secretion PNAS, November 27, 2007; 104(48): 19138 - 19143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Huang, D. R. Diener, A. Mitchell, G. J. Pazour, G. B. Witman, and J. L. Rosenbaum Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella J. Cell Biol., November 5, 2007; 179(3): 501 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hu, S. G. Wittekind, and M. M. Barr STAM and Hrs Down-Regulate Ciliary TRP Receptors Mol. Biol. Cell, September 1, 2007; 18(9): 3277 - 3289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Torayama, T. Ishihara, and I. Katsura Caenorhabditis elegans Integrates the Signals of Butanone and Food to Enhance Chemotaxis to Butanone J. Neurosci., January 24, 2007; 27(4): 741 - 750. [Abstract] [Full Text] [PDF]
|
|
