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A more recent version of this article appeared on May 1, 2006
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Submitted on November 4, 2005
Revised on February 13, 2006
Accepted on February 15, 2006
*Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205; Department of Molecular, Microbial, and Structural Biology, and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030
Monitoring Editor: Peter Walter
Mating yeast cells provide a genetically accessible system for the study of cell fusion. The dynamics of fusion pores between yeast cells were analyzed by following the exchange of fluorescent markers between fusion partners. On plasma membrane fusion, cytoplasmic GFP and DsRed diffuse between cells at rates proportional to the size of the fusion pore. GFP permeance measurements reveal that a typical fusion pore opens with a burst and then gradually expands. In some mating pairs, a sudden increase in GFP permeance was found, consistent with the opening of a second pore. In contrast, other fusion pores closed after permitting a limited amount of cytoplasmic exchange. Deletion of FUS1 from both mating partners caused a > 10-fold reduction in the initial permeance and expansion rate of the fusion pore. Although fus1 mating pairs also have a defect in degrading the cell wall that separates mating partners before plasma membrane fusion, other cell fusion mutants with cell wall remodeling defects had more modest effects on fusion pore permeance. Karyogamy is delayed by > 1 h in fus1 mating pairs, possibly as a consequence of retarded fusion pore expansion.
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