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MBC in Press, published online ahead of print August 2, 2006
Mol. Biol. Cell 10.1091/mbc.E05-11-1019

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Submitted on November 5, 2005
Revised on July 19, 2006
Accepted on July 26, 2006

Delineation of the Roles Played by RasG and RasC in cAMP-dependent Signal Transduction during the Early Development of Dictyostelium discoideum

Parvin Bolourani, George B. Spiegelman, and Gerald Weeks

Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada

Monitoring Editor: John Pringle

On starvation, the cellular slime mold Dictyostelium discoideum initiates a program of development leading to formation of multicellular structures. The initial cell aggregation requires chemotaxis to cyclic AMP (cAMP) and relay of the cAMP signal by the activation of adenylyl cyclase (ACA), and it has been shown previously that the Ras protein RasC is involved in both processes. Insertional inactivation of the rasG gene resulted in delayed aggregation and a partial inhibition of early gene expression, suggesting that RasG also has a role in early development. Both chemotaxis and ACA activation were reduced in the rasG- cells, but the effect on chemotaxis was more pronounced. When the responses of rasG- cells to cAMP were compared with the responses of rasC- and rasC- rasG- strains, generated in otherwise isogenic backgrounds, these studies revealed that signal transduction through RasG is more important in chemotaxis and early gene expression, but that signal transduction through RasC is more important in ACA activation. Because the loss of either of the two Ras proteins alone did not result in a total loss of signal output down either of the branches of the cAMP signal-response pathway, there appears to be some overlap of function.

Address correspondence to: Gerald Weeks (gerwee{at}interchange.ubc.ca)




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P. Bolourani, G. B. Spiegelman, and G. Weeks
Rap1 Activation in Response to cAMP Occurs Downstream of Ras Activation during Dictyostelium Aggregation
J. Biol. Chem., April 18, 2008; 283(16): 10232 - 10240.
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D. M. Veltman, I. Keizer-Gunnik, and P. J.M. Van Haastert
Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum
J. Cell Biol., February 25, 2008; 180(4): 747 - 753.
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