![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on September 1, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on December 7, 2005
Revised on June 19, 2006
Accepted on July 5, 2006
*Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205; Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria 3010, Australia
Monitoring Editor: Vivek Malhotra
In yeast, particular emphasis has been given to ER-derived, cisternal maturation models of Golgi assembly while in mammalian cells more emphasis has been given to golgins as a potentially stable assembly framework. In the case of de novo Golgi formation from the ER following brefeldin A/H89 washout in HeLa cells, we found that scattered, golgin-enriched, structures formed early and contained golgins including giantin, ranging across the entire cis to trans spectrum of the Golgi apparatus. These structures were incompetent in VSV-G cargo transport. Second, we compared Golgi competence in cargo transport to the kinetics of addition of various glycosyltransferases and glycosidases into nascent, golgin-enriched structures following drug washout. Enzyme accumulation was sequential with trans then medial glycosyltransferases/glycosidases found in the scattered, nascent Golgi. Involvement in cargo transport preceded full accumulation of enzymes or GPP130 into nascent Golgi. Third, during mitosis, we found that the formation of a golgin-positive acceptor compartment in early telophase preceded the accumulation of a Golgi glycosyltransferase in nascent Golgi structures. We conclude that during mammalian Golgi assembly components fit into a dynamic, first-formed, multi-golgin-enriched framework that is initially cargo transport incompetent. Resumption of cargo transport precedes full Golgi assembly.
This article has been cited by other articles:
|
|
 |
|
  Y. Wakana, S. Takai, K.-i. Nakajima, K. Tani, A. Yamamoto, P. Watson, D. J. Stephens, H.-P. Hauri, and M. Tagaya Bap31 Is an Itinerant Protein That Moves between the Peripheral Endoplasmic Reticulum (ER) and a Juxtanuclear Compartment Related to ER-associated Degradation Mol. Biol. Cell, May 1, 2008; 19(5): 1825 - 1836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sun, A. Shestakova, L. Hunt, S. Sehgal, V. Lupashin, and B. Storrie Rab6 Regulates Both ZW10/RINT-1 and Conserved Oligomeric Golgi Complex-dependent Golgi Trafficking and Homeostasis Mol. Biol. Cell, October 1, 2007; 18(10): 4129 - 4142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Boucrot and T. Kirchhausen Endosomal recycling controls plasma membrane area during mitosis PNAS, May 8, 2007; 104(19): 7939 - 7944. [Abstract] [Full Text] [PDF]
|
|
