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MBC in Press, published online ahead of print April 19, 2006
Mol. Biol. Cell 10.1091/mbc.E06-01-0080

A more recent version of this article appeared on July 1, 2006
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Submitted on January 27, 2006
Revised on April 6, 2006
Accepted on April 10, 2006

Rtn1p Is Involved in Structuring the Cortical ER

Johan-Owen De Craene,* Jeff Coleman,* Paula Estrada de Martin,{dagger} Marc Pypaert,* Scott Anderson,{ddagger} John R. Yates III,{ddagger} Susan Ferro-Novick,{dagger} and Peter Novick*

*Department of Cell Biology and {dagger}Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510; {ddagger}Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037

Monitoring Editor: Akihiko Nakano

The ER contains both cisternal and reticular elements in one contiguous structure. We identified rtn1{Delta} in a systematic screen for yeast mutants with altered ER morphology. The ER in rtn1{Delta} cells is predominantly cisternal rather than reticular, yet the net surface area of ER is not significantly changed. Rtn1-GFP associates with the reticular ER at the cell cortex and with the tubules that connect the cortical ER to the nuclear envelope, but not with the nuclear envelope itself. Rtn1p overexpression also results in an altered ER structure. Rtn proteins are found on the ER in a wide range of eukaryotes and are defined by two membrane-spanning domains flanking a conserved hydrophilic loop. Our results suggest that Rtn proteins may direct the formation of reticulated ER. We independently identified Rtn1p in a proteomic screen for proteins associated with the exocyst vesicle tethering complex. The conserved hydophilic loop of Rtn1p binds to the exocyst subunit Sec6p. Overexpression of this loop results in a modest accumulation of secretory vesicles suggesting impaired exocyst function. The interaction of Rtn1p with the exocyst at the bud tip may trigger the formation of a cortical ER network in yeast buds.

Address correspondence to: Peter Novick (peter.novick{at}yale.edu)




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