![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on December 1, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on February 3, 2006
Revised on September 8, 2006
Accepted on September 29, 2006
*Department of Genetics, Case Comprehensive Cancer Center, and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106
Monitoring Editor: Peter Walter
Recent advances in genome-wide analysis of alternative splicing indicate that extensive alternative RNA processing is associated with many proteins that play important roles in the nervous system. While differential splicing and polyadenylation make significant contributions to the complexity of the nervous system, our understanding of the regulatory mechanisms underlying the neuron-specific pathways is very limited. Mammalian neuron-specific ELAV-like Hu proteins (HuB, HuC and HuD) are a family of RNA-binding proteins implicated in neuronal differentiation and maintenance. It has been established that Hu proteins increase expression of proteins associated with neuronal function by up-regulating mRNA stability and/or translation in the cytoplasm. We report here a novel function of these proteins as RNA processing regulators in the nucleus. We further elucidate the underlying mechanism of this regulation. We show that in neuron-like cells, Hu proteins block the activity of TIA-1/TIAR, two previously identified, ubiquitously expressed proteins that promote the nonneuronal pathway of calcitonin/CGRP pre-mRNA processing. These studies define not only the first neuron-specific regulator of the calcitonin/CGRP system, but also the first nuclear function of Hu proteins.
This article has been cited by other articles:
|
|
 |
|
  H. Wang, J. Molfenter, H. Zhu, and H. Lou Promotion of exon 6 inclusion in HuD pre-mRNA by Hu protein family members Nucleic Acids Res., February 16, 2010; (2010) gkq028v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Barron, H. Zhu, M. N. Hinman, A. N. Ladd, and H. Lou The neurofibromatosis type I pre-mRNA is a novel target of CELF protein-mediated splicing regulation Nucleic Acids Res., January 1, 2010; 38(1): 253 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cherry, H. Jones, V. A. Karschner, and P. H. Pekala Post-transcriptional Control of CCAAT/Enhancer-binding Protein {beta} (C/EBP{beta}) Expression: FORMATION OF A NUCLEAR HuR-C/EBP{beta} mRNA COMPLEX DETERMINES THE AMOUNT OF MESSAGE REACHING THE CYTOSOL J. Biol. Chem., November 7, 2008; 283(45): 30812 - 30820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-L. Zhou and H. Lou Repression of Prespliceosome Complex Formation at Two Distinct Steps by Fox-1/Fox-2 Proteins Mol. Cell. Biol., September 1, 2008; 28(17): 5507 - 5516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Izquierdo Hu Antigen R (HuR) Functions as an Alternative Pre-mRNA Splicing Regulator of Fas Apoptosis-promoting Receptor on Exon Definition J. Biol. Chem., July 4, 2008; 283(27): 19077 - 19084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ratti, C. Fallini, C. Colombrita, A. Pascale, U. Laforenza, A. Quattrone, and V. Silani Post-transcriptional Regulation of Neuro-oncological Ventral Antigen 1 by the Neuronal RNA-binding Proteins ELAV J. Biol. Chem., March 21, 2008; 283(12): 7531 - 7541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhu, M. N. Hinman, R. A. Hasman, P. Mehta, and H. Lou Regulation of Neuron-Specific Alternative Splicing of Neurofibromatosis Type 1 Pre-mRNA Mol. Cell. Biol., February 15, 2008; 28(4): 1240 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. David and J. L. Manley The search for alternative splicing regulators: new approaches offer a path to a splicing code Genes & Dev., February 1, 2008; 22(3): 279 - 285. [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sathyan, H. B. Golden, and R. C. Miranda Competing Interactions between Micro-RNAs Determine Neural Progenitor Survival and Proliferation after Ethanol Exposure: Evidence from an Ex Vivo Model of the Fetal Cerebral Cortical Neuroepithelium J. Neurosci., August 8, 2007; 27(32): 8546 - 8557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Izquierdo and J. Valcarcel Two Isoforms of the T-cell Intracellular Antigen 1 (TIA-1) Splicing Factor Display Distinct Splicing Regulation Activities: CONTROL OF TIA-1 ISOFORM RATIO BY TIA-1-RELATED PROTEIN J. Biol. Chem., July 6, 2007; 282(27): 19410 - 19417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Coutinho-Mansfield, Y. Xue, Y. Zhang, and X.-D. Fu PTB/nPTB switch: a post-transcriptional mechanism for programming neuronal differentiation Genes & Dev., July 1, 2007; 21(13): 1573 - 1577. [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Voelker and J. A. Berglund A comprehensive computational characterization of conserved mammalian intronic sequences reveals conserved motifs associated with constitutive and alternative splicing Genome Res., July 1, 2007; 17(7): 1023 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-I G. Chen, R. E. Moore, H. Y. Ge, M. K. Young, T. D. Lee, and S. W. Stevens Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors Nucleic Acids Res., June 9, 2007; 35(12): 3928 - 3944. [Abstract] [Full Text] [PDF]
|
|
