Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 19, 2006
Mol. Biol. Cell 10.1091/mbc.E06-02-0143

A more recent version of this article appeared on October 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E06-02-0143v1
17/10/4364    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rida, P. C.G.
Right arrow Articles by Dean, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rida, P. C.G.
Right arrow Articles by Dean, N.

Submitted on February 16, 2006
Revised on July 11, 2006
Accepted on July 12, 2006

Yeast to Hyphal Transition Triggers Formin-dependent Golgi Localization to the Growing Tip in Candida albicans

Padmashree C.G. Rida,* Akiko Nishikawa,* Gena Y. Won, and Neta Dean

Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215

Monitoring Editor: Daniel Lew

Rapid and long distance secretion of membrane components is critical for hyphal formation in filamentous fungi, but the mechanisms responsible for polarized trafficking are not well understood. Here, we demonstrate that in Candida albicans, the majority of the Golgi complex is redistributed to the distal region during hyphal formation. Randomly distributed Golgi puncta in yeast cells cluster toward the growing tip during hyphal formation, remain associated with the distal portion of the filament during its extension, and are almost absent from the cell body. This restricted Golgi localization pattern is distinct from other organelles, including the ER, vacuole and mitochondria, which remain distributed throughout the cell body and hypha. Hyphal-induced positioning of the Golgi and the maintenance of its structural integrity requires actin cytoskeleton, but not microtubules. Absence of the formin Bni1 causes a hyphal-specific dispersal of the Golgi into a haze of finely dispersed vesicles with a sedimentation density no different from that of normal Golgi. These results demonstrate the existence of a hyphal-specific, Bni1-dependent cue for Golgi integrity and positioning at the distal portion of the hyphal tip, and suggest that filamentous fungi have evolved a novel strategy for polarized secretion, involving a redistribution of the Golgi to the growing tip.

*These authors contributed equally to this work.

Address correspondence to: Neta Dean (Neta.Dean{at}stonybrook.edu)




This article has been cited by other articles:


Home page
 MicrobiologyHome page
M. A. Hubbard and S. G. W. Kaminskyj
Rapid tip-directed movement of Golgi equivalents in growing Aspergillus nidulans hyphae suggests a mechanism for delivery of growth-related materials
Microbiology, May 1, 2008; 154(5): 1544 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Vjestica, X.-Z. Tang, and S. Oliferenko
The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast
Mol. Biol. Cell, March 1, 2008; 19(3): 1125 - 1138.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. J. Wolyniak and P. Sundstrom
Role of Actin Cytoskeletal Dynamics in Activation of the Cyclic AMP Pathway and HWP1 Gene Expression in Candida albicans
Eukaryot. Cell, October 1, 2007; 6(10): 1824 - 1840.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
G. Steinberg
Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper
Eukaryot. Cell, March 1, 2007; 6(3): 351 - 360.
[Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.