Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print April 12, 2006
Mol. Biol. Cell 10.1091/mbc.E06-03-0187

A more recent version of this article appeared on July 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-03-0187v1
17/7/2882    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tehrani, S.
Right arrow Articles by Cooper, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tehrani, S.
Right arrow Articles by Cooper, J. A.

Submitted on March 8, 2006
Accepted on April 4, 2006

Cortactin Has an Essential and Specific Role in Osteoclast Actin Assembly

Shandiz Tehrani,* Roberta Faccio,{dagger} Indra Chandrasekar,* F. Patrick Ross,{ddagger} and John A. Cooper*

Departments of *Cell Biology and Physiology, {dagger}Orthopedic Surgery, and {ddagger}Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110

Monitoring Editor: Thomas Pollard

Osteoclasts are essential for bone dynamics and calcium homeostasis. The cells form a tight seal on the bone surface, onto which they secrete acid and proteases to resorb bone. The seal is associated with a ring of actin filaments. Cortactin, a c-Src substrate known to promote Arp2/3-mediated actin assembly in vitro, is expressed in osteoclasts and localizes to the sealing ring. To address the role of cortactin and actin assembly in osteoclasts, we depleted cortactin by RNA interference. Cortactin-depleted osteoclasts displayed a complete loss of bone resorption with no formation of sealing zones. On nonosteoid surfaces, osteoclasts flatten with a dynamic, actin-rich peripheral edge that contains podosomes, filopodia and lamellipodia. Cortactin depletion led to a specific loss of podosomes, revealing a tight spatial compartmentalization of actin assembly. Podosome formation was restored in cortactin-depleted cells by expression of wildtype cortactin or an SH3 point mutant of cortactin. In contrast, expression of a cortactin mutant lacking tyrosine residues phosphorylated by Src did not restore podosome formation. Cortactin was found to be an early component of the nascent podosome belt, along with dynamin, supporting a role for cortactin in actin assembly.

Address correspondence to: John A. Cooper (jcooper{at}wustl.edu)




This article has been cited by other articles:


Home page
 Cancer Res.Home page
C. Tu, C. F. Ortega-Cava, G. Chen, N. D. Fernandes, D. Cavallo-Medved, B. F. Sloane, V. Band, and H. Band
Lysosomal Cathepsin B Participates in the Podosome-Mediated Extracellular Matrix Degradation and Invasion via Secreted Lysosomes in v-Src Fibroblasts
Cancer Res., November 15, 2008; 68(22): 9147 - 9156.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Dorfleutner, Y. Cho, D. Vincent, J. Cunnick, H. Lin, S. A. Weed, C. Stehlik, and D. C. Flynn
Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells
J. Cell Sci., July 15, 2008; 121(14): 2394 - 2405.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Badowski, G. Pawlak, A. Grichine, A. Chabadel, C. Oddou, P. Jurdic, M. Pfaff, C. Albiges-Rizo, and M. R. Block
Paxillin Phosphorylation Controls Invadopodia/Podosomes Spatiotemporal Organization
Mol. Biol. Cell, February 1, 2008; 19(2): 633 - 645.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
O. Destaing, A. Sanjay, C. Itzstein, W. C. Horne, D. Toomre, P. De Camilli, and R. Baron
The Tyrosine Kinase Activity of c-Src Regulates Actin Dynamics and Organization of Podosomes in Osteoclasts
Mol. Biol. Cell, January 1, 2008; 19(1): 394 - 404.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Chabadel, I. Banon-Rodriguez, D. Cluet, B. B. Rudkin, B. Wehrle-Haller, E. Genot, P. Jurdic, I. M. Anton, and F. Saltel
CD44 and beta3 Integrin Organize Two Functionally Distinct Actin-based Domains in Osteoclasts
Mol. Biol. Cell, December 1, 2007; 18(12): 4899 - 4910.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Tehrani, N. Tomasevic, S. Weed, R. Sakowicz, and J. A. Cooper
Src phosphorylation of cortactin enhances actin assembly
PNAS, July 17, 2007; 104(29): 11933 - 11938.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zhu, D. Yu, X.-C. Zeng, K. Zhou, and X. Zhan
Receptor-mediated Endocytosis Involves Tyrosine Phosphorylation of Cortactin
J. Biol. Chem., June 1, 2007; 282(22): 16086 - 16094.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. S. Clark, A. S. Whigham, W. G. Yarbrough, and A. M. Weaver
Cortactin Is an Essential Regulator of Matrix Metalloproteinase Secretion and Extracellular Matrix Degradation in Invadopodia
Cancer Res., May 1, 2007; 67(9): 4227 - 4235.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
L. I. Cosen-Binker and A. Kapus
Cortactin: The Gray Eminence of the Cytoskeleton.
Physiology, October 1, 2006; 21(5): 352 - 361.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.