Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print October 18, 2006
Mol. Biol. Cell 10.1091/mbc.E06-04-0368

A more recent version of this article appeared on November 1, 2006 Originally published as MBC in Press, 10.1091/mbc.E06-04-0368 on August 30, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E06-04-0368v2
E06-04-0368v3
17/11/4736    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, L.
Right arrow Articles by Brown, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, L.
Right arrow Articles by Brown, G. W.

Submitted on May 1, 2006
Revised on August 4, 2006
Accepted on August 16, 2006

A Survey of Essential Gene Function in the Yeast Cell Division Cycle

Lisa Yu,*{dagger} Lourdes Peña Castillo,{ddagger}{dagger} Sanie Mnaimneh,{ddagger}{dagger} Timothy R. Hughes,{ddagger}{dagger} and Grant W. Brown*{dagger}

*Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8; {ddagger}Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada M5G 1L6; {dagger}Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada M5S 3E1

Monitoring Editor: Trisha Davis

Mutations impacting specific stages of cell growth and division have provided a foundation for dissecting mechanisms that underlie cell cycle progression. We have undertaken an objective examination of the yeast cell cycle through flow cytometric analysis of DNA content in TetO7 promoter mutant strains representing 75% of all essential yeast genes. Over 65% of the strains displayed specific alterations in DNA content, suggesting that reduced function of an essential gene in most cases impairs progression through a specific stage of the cell cycle. Because of the large number of essential genes required for protein biosynthesis, G1 accumulation was the most common phenotype observed in our analysis. In contrast, relatively few mutants displayed S-phase delay, and most of these were defective in genes required for DNA replication or nucleotide metabolism. G2 accumulation appeared to arise from a variety of defects. In addition to providing a global view of the diversity of essential cellular processes that influence cell cycle progression, these data also provided predictions regarding the functions of individual genes: we identified four new genes involved in protein trafficking (NUS1, PHS1, PGA2, PGA3), and we found that CSE1 and SMC4 are important for DNA replication.

Address correspondence to: Timothy R. Hughes (t.hughes{at}utoronto.ca) or Grant W. Brown (grant.brown{at}utoronto.ca)




This article has been cited by other articles:


Home page
 BioinformaticsHome page
Z. Guo, Y. Li, X. Gong, C. Yao, W. Ma, D. Wang, Y. Li, J. Zhu, M. Zhang, D. Yang, et al.
Edge-based scoring and searching method for identifying condition-responsive protein protein interaction sub-network
Bioinformatics, August 15, 2007; 23(16): 2121 - 2128.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. V. Rosado, D. Kressler, and J. d. l. Cruz
Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
Nucleic Acids Res., June 13, 2007; (2007) gkm388v1.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.