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MBC in Press, published online ahead of print August 16, 2006
Mol. Biol. Cell 10.1091/mbc.E06-06-0492

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Submitted on June 5, 2006
Revised on July 18, 2006
Accepted on August 9, 2006

Invasion of Host Cells by Salmonella typhimurium Requires Focal Adhesion Kinase and p130Cas

Jing Shi and James E. Casanova

Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville, VA 22908-0732

Monitoring Editor: Ralph Isberg

Salmonella typhimurium colonizes the intestinal epithelium by injecting an array of effector proteins into host cells that induces phagocytic uptake of attached bacteria. However, the host molecules targeted by these effectors remain poorly defined. Here, we demonstrate that S. typhimurium induces formation of focal adhesion-like complexes at sites of bacterial attachment, and that both focal adhesion kinase (FAK) and the scaffolding protein p130Cas are required for Salmonella uptake. Entry of Salmonella into FAK-/- cells is dramatically impaired and can be restored to control levels by expression of wild type FAK. Surprisingly, reconstitution of bacterial internalization requires neither the kinase domain of FAK nor activation of c-Src, but does require a C-terminal PXXP motif through which FAK interacts with Cas. Infection of Cas-/- cells is also impaired, and reconstitution of invasiveness requires the central Cas YXXP repeat domain. The invasion defect in Cas-/- cells can be suppressed by overexpression of FAK, suggesting a functional link between FAK and Cas in the regulation of Salmonella invasion. Together, these findings reveal a novel role for focal adhesion proteins in the invasion of host cells by Salmonella.

Address correspondence to: James E. Casanova (jec9e{at}virginia.edu)




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E. C. Boyle, N. F. Brown, J. H. Brumell, and B. B. Finlay
Src homology domain 2 adaptors affect adherence of Salmonella enterica serovar Typhimurium to non-phagocytic cells
Microbiology, October 1, 2007; 153(10): 3517 - 3526.
[Abstract] [Full Text] [PDF]


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