Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print December 4, 2006
Mol. Biol. Cell 10.1091/mbc.E06-07-0612

A more recent version of this article appeared on February 1, 2007 Originally published as MBC in Press, 10.1091/mbc.E06-07-0612 on November 29, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E06-07-0612v1
E06-07-0612v2
18/2/581    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yen, W.-L.
Right arrow Articles by Klionsky, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yen, W.-L.
Right arrow Articles by Klionsky, D. J.

Submitted on July 19, 2006
Revised on November 1, 2006
Accepted on November 21, 2006

Atg27 Is Required for Autophagy-dependent Cycling of Atg9

Wei-Lien Yen, Julie E. Legakis, Usha Nair, and Daniel J. Klionsky

Life Sciences Institute, and Departments of Molecular, Cellular and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109

Monitoring Editor: Suresh Subramani

Autophagy is a catabolic pathway for the degradation of cytosolic proteins or organelles and is conserved among all eukaryotic cells. The hallmark of autophagy is the formation of double-membrane cytosolic vesicles, termed autophagosomes, which sequester cytoplasm; however, the mechanism of vesicle formation and the membrane source remain unclear. In the yeast S. cerevisiae, selective autophagy mediates the delivery of specific cargos to the vacuole, the analog of the mammalian lysosome. The transmembrane protein Atg9 cycles between the mitochondria and the pre-autophagosomal structure, which is the site of autophagosome biogenesis. Atg9 is thought to mediate the delivery of membrane to the forming autophagosome. Here, we characterize a second transmembrane protein Atg27 that is required for specific autophagy in yeast. Atg27 is required for Atg9 cycling, and shuttles between the pre-autophagosomal structure, mitochondria and the Golgi complex. These data support a hypothesis that multiple membrane sources supply the lipids needed for autophagosome formation.

Address correspondence to: Daniel J. Klionsky (klionsky{at}umich.edu)




This article has been cited by other articles:


Home page
 Phil Trans R Soc BHome page
J. A. K. W. Kiel
Autophagy in unicellular eukaryotes
Phil Trans R Soc B, March 12, 2010; 365(1541): 819 - 830.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Y. Nazarko, J.-C. Farre, and S. Subramani
Peroxisome Size Provides Insights into the Function of Autophagy-related Proteins
Mol. Biol. Cell, September 1, 2009; 20(17): 3828 - 3839.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Periyasamy-Thandavan, M. Jiang, P. Schoenlein, and Z. Dong
Autophagy: molecular machinery, regulation, and implications for renal pathophysiology
Am J Physiol Renal Physiol, August 1, 2009; 297(2): F244 - F256.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Sekito, T. Kawamata, R. Ichikawa, K. Suzuki, and Y. Ohsumi
Atg17 recruits Atg9 to organize the pre-autophagosomal structure
Genes Cells, May 1, 2009; 14(5): 525 - 538.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. He, M. Baba, Y. Cao, and D. J. Klionsky
Self-Interaction Is Critical for Atg9 Transport and Function at the Phagophore Assembly Site during Autophagy
Mol. Biol. Cell, December 1, 2008; 19(12): 5506 - 5516.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Kanki and D. J. Klionsky
Mitophagy in Yeast Occurs through a Selective Mechanism
J. Biol. Chem., November 21, 2008; 283(47): 32386 - 32393.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Z. Xie, U. Nair, and D. J. Klionsky
Atg8 Controls Phagophore Expansion during Autophagosome Formation
Mol. Biol. Cell, August 1, 2008; 19(8): 3290 - 3298.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
K. Tarassov, V. Messier, C. R. Landry, S. Radinovic, M. M. S. Molina, I. Shames, Y. Malitskaya, J. Vogel, H. Bussey, and S. W. Michnick
An in Vivo Map of the Yeast Protein Interactome
Science, June 13, 2008; 320(5882): 1465 - 1470.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
I. Monastyrska, C. He, J. Geng, A. D. Hoppe, Z. Li, and D. J. Klionsky
Arp2 Links Autophagic Machinery with the Actin Cytoskeleton
Mol. Biol. Cell, May 1, 2008; 19(5): 1962 - 1975.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ropolo, D. Grasso, R. Pardo, M. L. Sacchetti, C. Archange, A. L. Re, M. Seux, J. Nowak, C. D. Gonzalez, J. L. Iovanna, et al.
The Pancreatitis-induced Vacuole Membrane Protein 1 Triggers Autophagy in Mammalian Cells
J. Biol. Chem., December 21, 2007; 282(51): 37124 - 37133.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Yorimitsu, S. Zaman, J. R. Broach, and D. J. Klionsky
Protein Kinase A and Sch9 Cooperatively Regulate Induction of Autophagy in Saccharomyces cerevisiae
Mol. Biol. Cell, October 1, 2007; 18(10): 4180 - 4189.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2006 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.