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A more recent version of this article appeared on December 1, 2006 Originally published as MBC in Press, 10.1091/mbc.E06-07-0657 on October 18, 2006
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Submitted on August 1, 2006
Revised on September 7, 2006
Accepted on September 8, 2006
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210
Monitoring Editor: Mark Solomon
To define the extent of the modification of the nuclear pore complex (NPC) during Aspergillus nidulans closed mitosis a systematic analysis of nuclear transport genes has been completed. 30 genes have been deleted defining 12 nonessential and 18 essential genes. Several of the nonessential deletions caused conditional phenotypes and self sterility whereas deletion of some essential genes caused defects in nuclear structure. Live cell imaging of endogenously tagged NPC proteins (Nups) revealed that during mitosis 14 predicted peripheral Nups, including all FG repeat Nups, disperse throughout the cell. A core mitotic NPC structure consisting of membrane Nups, all components of the An-Nup84 subcomplex, An-Nup170 and, surprisingly, An-Gle1 remained throughout mitosis. We propose this minimal mitotic NPC core provides a conduit across the nuclear envelope and acts as a scaffold to which dispersed Nups return during mitotic exit. Further, unlike other dispersed Nups, An-Nup2 locates exclusively to mitotic chromatin suggesting it may have a novel mitotic role in addition to its nuclear transport functions. Importantly, its deletion causes lethality and defects in DNA segregation. This work defines the dramatic changes in NPC composition during A. nidulans mitosis and provides insight into how NPC disassembly may be integrated with mitosis.
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