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MBC in Press, published online ahead of print April 18, 2007
Mol. Biol. Cell 10.1091/mbc.E06-08-0738

A more recent version of this article appeared on July 1, 2007
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Submitted on August 22, 2006
Revised on March 29, 2007
Accepted on April 11, 2007

The Conserved Spc7 Protein Is Required for Spindle Integrity and Links Kinetochore Complexes in Fission Yeast

Anne Kerres,* Visnja Jakopec,* and Ursula Fleig

Lehrstuhl für Funktionelle Genomforschung der Mikroorganismen, Heinrich-Heine Universität, 40225 Düsseldorf, Germany

Monitoring Editor: Ted Salmon

Spc7, a member of the conserved Spc105/KNL-1 family of kinetochore proteins, was identified as an interaction partner of the EB1 homologue Mal3. Spc7 associates with the central centromere region of the chromosome but does not affect transcriptional silencing. Here, we show that Spc7 is required for the integrity of the spindle as well as for targeting of MIND but not of Ndc80 complex components to the kinetochore. Spindle defects in spc7 mutants were severe ranging from the inability to form a bipolar spindle in early mitosis to broken spindles in midanaphase B. spc7 mutant phenotypes were partially rescued by extra {alpha}-tubulin or extra Mal2. Thus, Spc7 interacts genetically with the Mal2-containing Sim4 complex.

*These authors contributed equally to this work.

Address correspondence to: Ursula Fleig (fleigu{at}uni-duesseldorf.de)






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