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MBC in Press, published online ahead of print December 20, 2006
Mol. Biol. Cell 10.1091/mbc.E06-08-0764

A more recent version of this article appeared on March 1, 2007
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Submitted on August 29, 2006
Revised on November 17, 2006
Accepted on December 1, 2006

The Unique-5 and -6 Motifs of ZO-1 Regulate Tight Junction Strand Localization and Scaffolding Properties

Alan S. Fanning,* Brent P. Little,{dagger} Christoph Rahner,{ddagger} Darkhan Utepbergenov,{sect} Zenta Walther,|| and James M. Anderson*

*Department of Cell and Molecular Physiology and {sect}School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7545; {dagger}Department of Diagnostic Radiology, Albert Einstein College of Medicine, Bronx, NY 10467; Departments of {ddagger}Cell Biology and ||Pathology, Yale University School of Medicine, New Haven, CT 06510

Monitoring Editor: Ben Margolis

The proper cellular location and sealing of tight junctions is assumed to depend on scaffolding properties of ZO-1, a member of the MAGUK protein family. ZO-1 contains a conserved SH3-GUK module that is separated by a variable region (unique-5), which in other MAGUKs has proven regulatory functions. To identify motifs in ZO-1 critical for its putative scaffolding functions, we focused on the SH3-GUK module including unique-5 (U5) and unique-6 (U6), a motif immediately C-terminal of the GUK domain. In vitro binding studies reveal U5 is sufficient for occludin binding; U6 reduces the affinity of this binding. In cultured cells, U5 is required for targeting ZO-1 to tight junctions and removal of U6 results in ectopically displaced junction strands containing the modified ZO-1, occludin and claudin on the lateral cell membrane. These results provide evidence that ZO-1 can control the location of tight junction transmembrane proteins and reveals complex protein binding and targeting signals within its SH3-U5-GUK-U6 region. We review these findings in the context of regulated scaffolding functions of other MAGUK proteins. Keywords: ZO-1/Occludin/tight junction/MAGUK/intramolecular

Address correspondence to: Alan S. Fanning (alan.fanning{at}med.unc.edu)




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