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MBC in Press, published online ahead of print January 31, 2007
Mol. Biol. Cell 10.1091/mbc.E06-09-0841

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Submitted on September 19, 2006
Revised on January 17, 2007
Accepted on January 22, 2007

Diversification of Function by Different Isoforms of Conventionally Shared RNA Polymerase Subunits

Sara Devaux,*{dagger} Steven Kelly,{dagger}{ddagger} Laurence Lecordier,* Bill Wickstead,{ddagger} David Perez-Morga,* Etienne Pays,* Luc Vanhamme,* and Keith Gull{ddagger}

*Institute for Molecular Biology and Medicine, Université Libre de Bruxelles, 6041 Gosselies, Belgium; {ddagger}Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom

Monitoring Editor: Wendy Bickmore

Eukaryotic nuclei contain three classes of multi-subunit DNA-directed RNA polymerase. At the core of each complex is a set of 12 highly conserved subunits of which 5 - RPB5, RPB6, RPB8, RPB10 and RPB12 - are thought to be common to all three polymerase classes. Here we show that four distantly related eukaryotic lineages (the higher plant and three protistan) have independently expanded their repertoire of RPB5 and RPB6 subunits. Using the protozoan parasite Trypanosoma brucei as a model organism we demonstrate that these distinct RPB5 and RPB6 subunits localize to discrete subnuclear compartments and form part of different polymerase complexes. We further show that RNAi-mediated depletion of these discrete subunits abolishes class specific transcription and hence demonstrate complex specialization and diversification of function by conventionally shared subunit groups.

{dagger}These authors contributed equally to this work.

Address correspondence to: Luc Vanhamme (luc.vanhamme{at}ulb.ac.be) or Keith Gull (keith.gull{at}path.ox.ac.uk)




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