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MBC in Press, published online ahead of print March 28, 2007
Mol. Biol. Cell 10.1091/mbc.E06-10-0918

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Submitted on October 13, 2006
Revised on March 12, 2007
Accepted on March 19, 2007

Novel sfi1 Alleles Uncover Additional Functions for Sfi1p in Bi-Polar Spindle Assembly and Function

Victoria E. Anderson,* John Prudden,*{dagger} Simon Prochnik,*{ddagger} Thomas H. Giddings Jr.,{sect} and Kevin G. Hardwick*

*Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3JR, United Kingdom; {sect}Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309

Monitoring Editor: Tim Stearns

A variety of spindle and kinetochore defects have been shown to induce a mitotic delay through activation of the spindle checkpoint. With the aim of identifying novel mitotic defects we carried out a mad1 synthetic lethal screen in budding yeast. In this screen four novel alleles of sfi1 were isolated. SFI1 is an essential gene, previously identified through its interaction with centrin/CDC31 and shown to be required for spindle pole body (SPB) duplication. The new mutations were all found in the C-terminal domain of Sfi1p, which has no known function but is well conserved among budding yeasts. Analysis of the novel sfi1 mutants, through a combination of light and electron microscopy, revealed duplicated SPBs <0.3 µm apart. Importantly, these SPBs have completed duplication but are not separated, suggesting a possible defect in splitting of the bridge. We discuss possible roles for Sfi1p in this step in bipolar spindle assembly.

Present addresses: {dagger}Medical Research Council Radiation and Genome Stability Unit, Harwell, Oxon, United Kingdom; {ddagger}Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598.

Address correspondence to: Kevin G. Hardwick (Kevin.Hardwick{at}ed.ac.uk)




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