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MBC in Press, published online ahead of print April 11, 2007
Mol. Biol. Cell 10.1091/mbc.E06-10-0959

A more recent version of this article appeared on June 1, 2007
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Submitted on October 27, 2006
Revised on March 19, 2007
Accepted on March 30, 2007

Direct Observation of Regulated RNP Transport across the Nurse Cell/Oocyte Boundary

Sarah Mische, Mingang Li, Madeline Serr, and Thomas S. Hays

Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455

Monitoring Editor: Susan Wente

In Drosophila, the asymmetric localization of specific mRNAs to discrete regions within the developing oocyte determines the embryonic axes. The microtubule motors, dynein and kinesin, are required for the proper localization of the determinant ribonucleoprotein complexes (RNPs), but the mechanisms that account for RNP transport to and within the oocyte are not well understood. In this work, we focus on the transport of RNA complexes containing bicoid (bcd), an anterior determinant. We show in live egg chambers that, within the nurse cell compartment, dynein actively transports GFP-tagged Exuperantia, a cofactor required for bcd RNP localization. Surprisingly, the loss of kinesin I activity elevates RNP motility in nurse cells, while disruption of dynein activity inhibits RNP transport. Once RNPs are transferred through the ring canal to the oocyte, they no longer display rapid, linear movements, but are distributed by cytoplasmic streaming and gradually disassemble. By contrast, bcd mRNA injected into oocytes assembles de novo into RNP particles that exhibit rapid, dynein-dependent transport. We speculate that after delivery to the oocyte, RNP complexes may disassemble and be remodeled with appropriate accessory factors to ensure proper localization.

Address correspondence to: Thomas S. Hays (haysx001{at}umn.edu)






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