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MBC in Press, published online ahead of print January 10, 2007
Mol. Biol. Cell 10.1091/mbc.E06-10-0974

A more recent version of this article appeared on March 1, 2007
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Submitted on November 2, 2006
Revised on December 21, 2006
Accepted on January 2, 2007

VAMP8/Endobrevin as a General v-SNARE for Regulated Exocytosis of the Exocrine System

Cheng-Chun Wang,* Hong Shi,* Ke Guo,* Chenpeng Ng,* Jia Li,* Bin Qi Gan,* Hwee Chien Liew,* Jukka Leinonen,{dagger} Hannu Rajaniemi,{dagger} Zhi Hong Zhou,* Qi Zeng,* and Wanjin Hong*

*Institute of Molecular and Cell Biology, Singapore 138673, Singapore; {dagger}Department of Anatomy and Cell Biology, University of Oulu, 90014 Oulu, Finland

Monitoring Editor: Vivek Malhotra

The molecular mechanism governing the regulated secretion of most exocrine tissues remains elusive, although VAMP8/endobrevin has recently been shown to be the major v-SNARE of zymogen granules of pancreatic exocrine acinar cells. In this paper, we characterized the role of VAMP8 in the entire exocrine system. Immunohistochemical studies showed that VAMP8 is expressed in all examined exocrine tissues such as salivary glands, lacrimal (tear) glands, sweat glands, sebaceous glands, mammary glands and the prostate. Severe anomalies were observed in the salivary and lacrimal glands of VAMP8-null mice. Mutant salivary glands accumulated amylase and carbonic anhydrase VI. Electron microscopy revealed an accumulation of secretory granules in the acinar cells of mutant parotid and lacrimal glands. Pilocarpine-stimulated secretion of saliva proteins was compromised in the absence of VAMP8. Protein aggregates were observed in mutant lacrimal glands. VAMP8 may interact with syntaxin 4 and SNAP-23. These results suggest that VAMP8 may act as a v-SNARE for regulated secretion of the entire exocrine system.

Address correspondence to: Wanjin Hong (mcbhwj{at}imcb.a-star.edu.sg)




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