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MBC in Press, published online ahead of print April 18, 2007
Mol. Biol. Cell 10.1091/mbc.E06-12-1128

A more recent version of this article appeared on July 1, 2007
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Submitted on December 19, 2006
Revised on April 2, 2007
Accepted on April 9, 2007

MTOC Reorientation Occurs during Fc{gamma}R-mediated Phagocytosis in Macrophages

Edward W. Eng,*{dagger} Adam Bettio,{dagger} John Ibrahim,{dagger} and Rene E. Harrison*{dagger}

Departments of *Cell and Systems Biology and {dagger}Biological Sciences, University of Toronto at Scarborough, Toronto, Ontario M1C 1A4, Canada

Monitoring Editor: Patrick Brennwald

Cell polarization is essential for targeting signaling elements and organelles to active plasma membrane regions. In a few specialized cell types, cell polarity is enhanced by reorientation of the MTOC and associated organelles toward dynamic membrane sites. Phagocytosis is a highly polarized process whereby particles >0.5 µm are internalized at stimulated regions on the cell surface of macrophages. Here we provide detailed evidence that the MTOC reorients toward the site of particle internalization during phagocytosis. We visualized MTOC proximity to IgG-sRBCs in fixed RAW264.7 cells, during live cell imaging using fluorescent chimeras to label the MTOC and using frustrated phagocytosis assays. MTOC reorientation in macrophages is initiated by Fc{gamma}R-ligation and is complete within one hour. Polarization of the MTOC toward the phagosome requires the MT cytoskeleton and dynein motor activity. cdc42, PI3K and mPAR-6 are all important signaling molecules for MTOC reorientation during phagocytosis. MTOC reorientation was not essential for particle internalization or phagolysosome formation. However Golgi reorientation in concert with MTOC reorientation during phagocytosis implicates MTOC reorientation in antigen processing events in macrophages.

Address correspondence to: Rene E. Harrison (harrison{at}utsc.utoronto.ca)




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