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A more recent version of this article appeared on July 1, 2007
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Submitted on December 26, 2006
Revised on April 9, 2007
Accepted on April 19, 2007
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721-0106
Monitoring Editor: Thomas Fox
Recent experiments have shown that mRNAs can move between polysomes and P-bodies, which are aggregates of nontranslating mRNAs associated with translational repressors and the mRNA decapping machinery. The transitions between polysomes and P-bodies and how the poly(A) tail and the associated poly(A) binding protein (Pab1p) may affect this process are unknown. Herein, we provide evidence that poly(A)+ mRNAs can enter P-bodies in yeast. First, we show that both poly(A)- and poly(A)+ mRNA become translationally repressed during glucose deprivation, where mRNAs accumulate in P-bodies. In addition, both poly(A)+ transcripts and/or Pab1p can be detected in P-bodies during glucose deprivation and in stationary phase. Cells lacking Pab1p have enlarged P-bodies suggesting that Pab1p plays a direct or indirect role in shifting the equilibrium of mRNAs away from P-bodies and into translation, perhaps by aiding in the assembly of a type of mRNP within P-bodies that is poised to reenter translation. Consistent with this latter possibility, we observed the translation initiation factors eIF4E and eIF4G in P-bodies at a low level during glucose deprivation and at high levels in stationary phase. Moreover, Pab1p exited P-bodies much faster than Dcp2p when stationary phase cells were given fresh nutrients. Taken together, these results suggest that polyadenylated mRNAs can enter P-bodies, and an mRNP complex including poly(A)+ mRNA, Pab1p, eIF4E and eIF4G2 may represent a transition state during the process of mRNAs exchanging between P-bodies and translation.
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