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MBC in Press, published online ahead of print August 8, 2007
Mol. Biol. Cell 10.1091/mbc.E07-01-0085

A more recent version of this article appeared on October 1, 2007
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Submitted on January 31, 2007
Revised on July 24, 2007
Accepted on August 1, 2007

Abl Tyrosine Kinase Promotes Dorsal Ruffles but Restrains Lamellipodia Extension during Cell Spreading on Fibronectin

Hua Jin* and Jean Y.J. Wang*{dagger}{ddagger}

*Division of Biological Sciences, {dagger}Division of Hematology-Oncology, Department of Medicine, School of Medicine, and {ddagger}Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093-0820

Monitoring Editor: Richard Assoian

The nonreceptor Abl tyrosine kinase stimulates F-actin microspikes and membrane ruffles in response to adhesion and growth factor signals. We show here that induced dimerization of Abl-FKBP, but not the kinase-defective AblKD-FKBP, inhibits cell spreading on fibronectin. Conversely, knockdown of cellular Abl by shRNA stimulates cell spreading. The Abl kinase inhibitor, imatinib, also stimulates cell spreading and its effect is overridden by the imatinib-resistant AblT315I. Expression of Abl but not AbkKD in Abl/Arg-deficient cells again inhibits spreading. Furthermore, Abl inhibits spreading of cells that express the activated Rac, RacV12, correlating with RacV12 localization to dorsal membrane protrusions. Ectopic expression of CrkII, a Rac activator that is inactivated by Abl-mediated tyrosine phosphorylation, antagonizes Abl-mediated dorsal membrane localization of RacV12. Ectopic expression of a dynamin-2 mutant, previously shown to induce Rac-GTP localiazation to the dorsal membrane, abolishes the stimulatory effect of imatinib on cell spreading. These results suggest that Abl tyrosine kinase, through CrkII phosphorylation and in collaboration with dynamin-2 can regulate the partitioning of Rac-GTP to favor dorsal ruffles during cell spreading. The Abl-dependent dorsal membrane localization of activated Rac explains its positive role in ruffling and negative role in cell spreading and migration.

Address correspondence to: Jean Y.J. Wang (jywang{at}ucsd.edu)




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