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MBC in Press, published online ahead of print June 20, 2007
Mol. Biol. Cell 10.1091/mbc.E07-02-0146

A more recent version of this article appeared on September 1, 2007
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Submitted on February 20, 2007
Revised on May 30, 2007
Accepted on June 13, 2007

The Concentration of Nuf, a Rab11 Effector, at the MTOC Is Cell Cycle-regulated, Dynein-dependent, and Coincides with the Timing of Furrow Formation in the Early Drosophila Embryo

Blake Riggs,*{dagger} Barbara Fasulo,*{dagger} Anne Royou,* Sarah Mische,{ddagger} Jian Cao,* Thomas S. Hays,{ddagger} and William Sullivan*

*Sinsheimer Laboratories, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, CA 95064; {ddagger}Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55108-1095

Monitoring Editor: Yu-li Wang

Animal cytokinesis relies on membrane addition as well as acto-myosin based constriction. Recycling Endosome (RE)-derived vesicles are a key source of this membrane. Rab11, a small GTPase associated with the RE and involved in vesicle targeting, is required for elongation of the cytokinetic furrow. In the early Drosophila embryo, Nuclear-fallout (Nuf), a Rab11 effector, promotes vesicle-mediated membrane delivery and actin organization at the invaginating furrow. While Rab11 maintains a relatively constant localization at the MTOC, Nuf is present at the MTOC only during the phases of the cell cycle in which furrow invagination occurs. We demonstrate that Nuf protein levels remain relatively constant throughout the cell cycle suggesting that Nuf is undergoing cycles of concentration and dispersion from the MTOC. Microtubules, but not microfilaments, are required for proper MTOC localization of Nuf and Rab11. The MTOC localization of Nuf also relies on Dynein. Immunoprecipitation experiments demonstrate that Nuf and Dynein physically interact. In accord with these findings, and in contrast to previous reports, we demonstrate that microtubules are required for proper metaphase furrow formation. We propose that the cell cycle regulated, Dynein-dependent recruitment of Nuf to the MTOC influences the timing of RE-based vesicle delivery to the invaginating furrows.

{dagger}These authors contributed equally to this work.

Address correspondence to: William Sullivan (sullivan{at}biology.ucsc.edu)




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