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MBC in Press, published online ahead of print April 11, 2007
Mol. Biol. Cell 10.1091/mbc.E07-02-0154

A more recent version of this article appeared on June 1, 2007
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Submitted on February 20, 2007
Accepted on April 2, 2007

The Nucleolar Channel System of Human Endometrium Is Related to Endoplasmic Reticulum and R-Rings

Nupur Kittur,* Gregory Zapantis,{dagger} Mira Aubuchon,{dagger} Nanette Santoro,{dagger} David P. Bazett-Jones,{ddagger} and U. Thomas Meier*

Departments of *Anatomy and Structural Biology and {dagger}Obstetrics, Gynecology, and Women’s Health, Albert Einstein College of Medicine, Bronx, NY 10461; {ddagger}The Hospital for Sick Children, Toronto, ON M5G 1L7, Canada

Monitoring Editor: A. Gregory Matera

The nucleolar channel system (NCS) is a well-established ultrastructural hallmark of the post-ovulation endometrium. Its transient presence has been associated with human fertility. Nevertheless, the biogenesis, composition, and function of these intranuclear membrane cisternae are unknown. Membrane systems with a striking ultrastructural resemblance to the NCS, termed R-rings, are induced in nuclei of tissue culture cells by overexpression of the central repeat domain of the nucleolar protein Nopp140. Here we provide a first molecular characterization of the NCS and compare the biogenesis of these two enigmatic organelles. Like the R-rings, the NCS consists of endoplasmic reticulum harboring the marker glucose-6-phosphatase. R-ring formation initiates at the nuclear envelope, apparently by a calcium-mediated Nopp140-membrane interaction, as supported by the calcium binding ability of Nopp140, the inhibition of R-ring formation by calcium chelators, and by the concentration of Nopp140 and complexed calcium in R-rings. Although biogenesis of the NCS may initiate similarly, the reduced presence of complexed calcium and Nopp140 suggests the involvement of additional factors.

Address correspondence to: U. Thomas Meier (meier{at}aecom.yu.edu)




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E. Guffanti, N. Kittur, Z. N. Brodt, A. J. Polotsky, S. M. Kuokkanen, D. S. Heller, S. L. Young, N. Santoro, and U. T. Meier
Nuclear pore complex proteins mark the implantation window in human endometrium
J. Cell Sci., June 15, 2008; 121(12): 2037 - 2045.
[Abstract] [Full Text] [PDF]


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