Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print May 2, 2007
Mol. Biol. Cell 10.1091/mbc.E07-02-0167

A more recent version of this article appeared on July 1, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow All Versions of this Article:
E07-02-0167v1
18/7/2619    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van der Giessen, K.
Right arrow Articles by Gallouzi, I.-E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van der Giessen, K.
Right arrow Articles by Gallouzi, I.-E.

Submitted on February 23, 2007
Revised on April 23, 2007
Accepted on April 24, 2007

Involvement of Transportin 2-mediated HuR Import in Muscle Cell Differentiation

Kate van der Giessen and Imed-Eddine Gallouzi

Department of Biochemistry, McGill University, Montreal, Quebec, H3G 1Y6, Canada

Monitoring Editor: Marvin P. Wickens

Muscle fiber formation requires the sequential expression of myogenic regulatory factors (MRFs) such as MyoD and myogenin. The messenger RNAs encoding these two proteins are regulated posttranscriptionally through their ability to associate with the RNA-binding protein HuR. HuR localizes first to the nucleus, then to the cytoplasm during muscle differentiation. Therefore, we examined the link between this localization and the promyogenic function of HuR. We show that early in muscle differentiation, HuR is localized to the nucleus of myoblasts by active Transportin 2 (TRN2)-mediated import. In differentiated muscle fibers, however, the TRN2-HuR complex is disrupted, leading to the cytoplasmic localization of HuR, as well as to the stabilization of MyoD and myogenin mRNAs. Interrupting the TRN2-HuR complex using RNA interference against TRN2, or the cell-permeable peptides (AP) fused to the nucleocytoplasmic shuttling domain (HNS) of HuR, enhanced the efficiency of myofiber formation. Together, our data suggest that HuR import is disrupted in differentiated muscle fibers and this event constitutes an important regulatory step during myogenesis.

Address correspondence to: Imed-Eddine Gallouzi (imed.gallouzi{at}mcgill.ca)




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
R. Mazroui, S. Di Marco, E. Clair, C. von Roretz, S. A. Tenenbaum, J. D. Keene, M. Saleh, and I.-E. Gallouzi
Caspase-mediated cleavage of HuR in the cytoplasm contributes to pp32/PHAP-I regulation of apoptosis
J. Cell Biol., January 10, 2008; 180(1): 113 - 127.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2007 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.