![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on August 1, 2007
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on February 23, 2007
Revised on April 16, 2007
Accepted on May 7, 2007
¶
Departments of *Surgery, Cell and Developmental Biology, and Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232-2733; ¶Nashville Veterans Affairs Medical Center, Nashville, TN 37212-2637; Institute of Biotechnology, University of Helsinki, FIN-00014 Helsinki, Finland; ||Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198
Monitoring Editor: Patrick Brennwald
Cells utilize multiple pathways to internalize and recycle cell surface components. While Rab11a and Myosin Vb are involved in the recycling of proteins internalized by clathrin-mediated endocytosis, Rab8a has been implicated in nonclathrin-dependent endocytosis and recycling. By yeast two-hybrid assays, we have now demonstrated that Myosin Vb can interact with Rab8a, but not Rab8b. We have confirmed the interaction of Myosin Vb with Rab11a and Rab8a in vivo using fluorescent resonant energy transfer (FRET) techniques. Rab8a and Myosin Vb colocalize to a tubular network containing EHD1 and EHD3, which does not contain Rab11a. Myosin Vb tail can cause the accumulation of both Rab11a and Rab8a in collapsed membrane cisternae, while dominant-negative Rab11-FIP2(129-512) selectively accumulates Rab11a, but not Rab8a. Additionally, dynamic live cell imaging demonstrates distinct pathways for Rab11a and Rab8a vesicle trafficking. These findings indicate that Rab8a and Rab11a define different recycling pathways that both employ Myosin Vb.
This article has been cited by other articles:
|
|
 |
|
  S. Ishikura and A. Klip Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation Am J Physiol Cell Physiol, October 1, 2008; 295(4): C1016 - C1025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Henry and D. R. Sheff Rab8 Regulates Basolateral Secretory, But Not Recycling, Traffic at the Recycling Endosome Mol. Biol. Cell, May 1, 2008; 19(5): 2059 - 2068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Chibalina, M. N. J. Seaman, C. C. Miller, J. Kendrick-Jones, and F. Buss Myosin VI and its interacting protein LMTK2 regulate tubule formation and transport to the endocytic recycling compartment J. Cell Sci., December 15, 2007; 120(24): 4278 - 4288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Ducharme, J. A. Williams, A. Oztan, G. Apodaca, L. A. Lapierre, and J. R. Goldenring Rab11-FIP2 regulates differentiable steps in transcytosis Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1059 - C1072. [Abstract] [Full Text] [PDF]
|
|
