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MBC in Press, published online ahead of print June 20, 2007
Mol. Biol. Cell 10.1091/mbc.E07-03-0239

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Submitted on March 14, 2007
Revised on May 18, 2007
Accepted on June 13, 2007

STAM and Hrs Down-Regulate Ciliary TRP Receptors

Jinghua Hu,* Samuel G. Wittekind,{dagger} and Maureen M. Barr*

*Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin, Madison, WI 53705; {dagger}University of Washington School of Medicine, Seattle, WA

Monitoring Editor: Jean Gruenberg

Cilia are endowed with membrane receptors, channels, and signaling components whose localization and function must be tightly controlled. In primary cilia of mammalian kidney epithelia and sensory cilia of C. elegans neurons, polycystin-1 (PC1) and transient receptor polycystin-2 channel (TRPP2 or PC2), function together as a mechanosensory receptor-channel complex. Despite the importance of the polycystins in sensory transduction, the mechanisms that regulate polycystin activity and localization, or ciliary membrane receptors in general, remain poorly understood. We demonstrate that signal transduction adaptor molecule STAM-1A interacts with C. elegans LOV-1 (PC1), and that STAM functions with hepatocyte growth factor regulated tyrosine kinase substrate (Hrs) on early endosomes to direct the LOV-1-PKD-2 complex for lysosomal degradation. In a stam-1 mutant, both LOV-1 and PKD-2 improperly accumulate at the ciliary base. Conversely, overexpression of STAM or Hrs promotes the removal of PKD-2 from cilia, culminating in sensory behavioral defects. These data reveal that the STAM-Hrs complex, which downregulates ligand-activated growth factor receptors from the cell surface of yeast and mammalian cells, also regulates the localization and signaling of a ciliary PC1 receptor-TRPP2 complex.

Address correspondence to: Maureen M. Barr (mmbarr{at}pharmacy.wisc.edu)




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