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A more recent version of this article appeared on December 1, 2007
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Submitted on May 7, 2007
Revised on September 19, 2007
Accepted on September 25, 2007
Istituto Pasteur-Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche, Dipartimento di Genetica e Biologia Molecolare, Università di Roma "La Sapienza," 00185 Roma, Italy
Monitoring Editor: Fred Chang
Rab11 is a small GTPase that regulates several aspects of vesicular trafficking. Here we show that Rab11 accumulates at the cleavage furrow of Drosophila spermatocytes and is essential for cytokinesis. Mutant spermatocytes form regular actomyosin rings, but these rings fail to constrict to completion, leading to cytokinesis failures. rab11 spermatocytes also exhibit an abnormal accumulation of Golgi-derived vesicles at the telophase equator, suggesting a defect in membrane-vesicle fusion. These cytokinesis phenotypes are identical to those elicited by mutations in giotto (gio) and four wheel drive (fwd) that encode a PITP and a PtdIns 4-kinase, respectively. Double mutant analysis and immunostaining for Gio and Rab11 indicated that gio, fwd and rab11 function in the same cytokinetic pathway, with Gio and Fwd acting upstream of Rab11. We propose that Gio and Fwd mediate Rab11 recruitment at the cleavage furrow, and that Rab11 facilitates targeted membrane delivery to the advancing furrow.
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